高效液相色谱法快速检测乳饮料中常用甜味剂  

Rapid Determination of Common Sweeteners in Milk Beverage by HPLC

作　　者：鲁琳[1,2] 杭义萍[1] 高燕红[2] 

机构地区：[1]华南理工大学化学与化工学院,广东广州510640 [2]广东省疾病预防控制中心,广东广州510300

出　　处：《食品科学》2009年第10期166-168,共3页Food Science

基　　金：广东省医学科学技术研究基金项目(A2007070)

摘　　要：建立一种快速检测乳饮料中常用甜味剂乙酰磺胺酸钾、糖精钠、甜蜜素、阿斯巴甜、阿力甜的高效液相色谱方法。样品经过稀释、沉淀蛋白、高速离心等前处理后,采用SymmetryC18色谱柱分离,磷酸盐缓冲液(pH5.0)与乙腈为流动相,进行梯度洗脱,紫外检测波长为205nm。一次进样分析时间为12min,5种甜味剂均能很好分离,RSD为2.1%～5.6%(n=6),平均加标回收率为86.0%～102%。该方法简单、快速,重现性和选择性好,适用于快速检测乳饮料中的常用甜味剂。To establish a rapid analysis method for determination of common sweeteners such as acesulfame potassium, saccharin sodium, sodium cyclamate, aspavtame, and alitame in milk beverage by HPLC. After the pretreatments of dilution, protein precipitation, and high-speed centrifugadon, the sample was separated by Symmetry C18 column with phosphate buffer （pH 5.0） and acetonitrile as mobile phase, eluted gradiently, and detected at the UV wavelength of 205 rim. The analysis of one sample costs 12 rain, and all five sweeteners could be well separated. The average spike recoveries of the above five sweeteners were 86.0% to 102%, and the RSDs were 2.1% to 5.6% （n = 6）. This method is simple, quick, and of good reproducibility and selectivity, therefore it is an effective way to determine sweeteners in milk drink.

关 键 词：甜味剂 乙酰磺胺酸钾 糖精钠 甜蜜素 阿斯巴甜 阿力甜 高效液相色谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]