超高效液相色谱法测定油脂中脂肪酸的组成与分布  

Determination of fatty acid composition and distribution in oils by ultra-high performance liquid chromatography

作　　者：赵玉生[1] 陈翔[1,2] 王瑛瑶[2] 张蕊[2] 

机构地区：[1]郑州轻工业学院食品工程系,郑州450002 [2]国家粮食局科学研究院,北京100037

出　　处：《中国油脂》2009年第5期64-68,共5页China Oils and Fats

基　　金：公益性科研院所基金项目

摘　　要：以ω-溴苯乙酮作为脂肪酸衍生剂,利用超高效液相色谱(UPLC)法测定双低菜籽油和结构脂质中脂肪酸组成与位置分布,探讨了流动相、柱温对脂肪酸衍生物分离检测效果的影响。经优化确定以(甲醇-乙腈)(90∶10)-水为流动相梯度洗脱,在检测波长246 nm,柱温35℃,流速0.35mL/min的色谱条件下,18 min内能完成对13种脂肪酸衍生物的分离检测。UPLC法具有操作简单,分析检测时间短,溶剂消耗少,分离度好的优点。A method was developed for the determination of fatty acids positional distribution in triacylg-lycerols of double - low rapeseed oil and structured lipid using w-bromoacetophenone as derivative reagent by ultra -high performance liquid chromatography（ UPLC ）. Different chromatographic parameters ineluding elution profiles, column temperature were investigated in order to optimize separation conditions of different fatty acid phenaeyl ester derivatives. A gradient elution using methanol/acetonitrile （90 ： 10, V/V） -water as mobile phase at 0.35 mL/min（ flow velocity）, detection wavelength at 246 nm, and column temperature of 35℃ were chosen. Under these conditions, 13 derivatives of fatty acids were separated in 18 min. This method was simple, fast,little solvent-consuming and good separation degree.

关 键 词：超高效液相色谱 脂肪酸 双低菜籽油 结构脂质 

分 类 号：TQ646[化学工程—精细化工]