马铃薯淀粉磷酸寡糖的全酶法制备及其分离  

Study on the Enzymatic Preparation and Separation of Potato Phosphoryl Oligosaccharides

作　　者：朱培蕾[1,2] 汪名春 刘霞[2] 刘才宇[1] 赵贵云[1] 杜先锋[2] 

机构地区：[1]安徽省农业科学院园艺研究所,安徽合肥230031 [2]安徽农业大学食品科学与工程系,安徽合肥230036 [3]南京农业大学食品科学与技术学院,江苏南京210095

出　　处：《食品与发酵工业》2009年第5期74-78,共5页Food and Fermentation Industries

基　　金：国家863项目资助(No.2006AA10Z340)

摘　　要：研究了制备低葡萄糖当量值(dextrose equivalent value,DE)麦芽低聚糖的工艺,以及从中分离制备磷酸寡糖(phosphoryl oligosaccharides,POs)的工艺条件。以马铃薯淀粉为原料,采用全酶法结合喷射液化工艺制备低DE值麦芽低聚糖,采用高效液相色谱法(high performance liquid chromatography,HPLC)测定样品糖分组成,柱层析法从中分离制备磷酸寡糖,并利用红外光谱进行定性分析。结果表明,麦芽低聚糖液化DE值为15%,糖化DE值36%,其中含有葡萄糖及麦芽二至七糖,以麦芽二至五糖居多,符合分离制备磷酸寡糖的要求。弱碱性阴离子交换树脂HJ-30分离磷酸寡糖的工艺条件:洗脱液NaCl浓度0.5moL/L,洗脱速度1.2mL/min。红外光谱分析结果显示,样品为结合有磷酸基团的糖类物质。The paper studied on the crafts to prepare maltooligosaccharides with low dextrose equivalent （DE） values and phosphoryl oligosaccharides （POs）. Firstly, the low DE maltooligosaccharides was prepared from potato starch by enzymatic-jet liquefaction methodologies and was analyzed by high performance liquid chromatography （HPLC）. Then, POs was separated from maltooligosaccharides by column chromatography and analyzed by infrared chromatography. The results showed that the DE values of liquefie.d and saccharified maltooligosaccharides were 15% and 36%, respectively. HPLC analysis showed that it mainly contained maltoolgosaccharide with DP 2 to 5. The optimal conditions to separate POs by HJ- 30 column are as follows： the column.was eluted with 0.5 mol/L NaCl at 1.2 mL/min. IR analysis of POs showed that it was sugar with phosphate groups.

关 键 词：马铃薯淀粉 全酶法 磷酸寡糖 柱层析 分离 制备 

分 类 号：TS201.2[轻工技术与工程—食品科学]