香菇蛋白的提取制备方法研究  

Extraction and preparation methods of protein from Lentinus edodes

作　　者：李波[1] 朱静[1] 程静[1] 赵森[1] 田燕[1] 

机构地区：[1]河南科技学院食品学院,河南新乡453003

出　　处：《食品工业科技》2009年第6期226-228,231,共4页Science and Technology of Food Industry

基　　金：河南省高校新世纪优秀人才支持计划(2006HANCET-17);河南科技学院重点科研项目资助基金

摘　　要：利用香菇中蛋白和多糖溶解性的差异，首先将蛋白提取出来，残余物再用来提取多糖，并对香菇蛋白的提取和沉淀方法进行了研究。结果显示，香菇蛋白的提取制备方法是：香菇粉中加入10-20倍pH为10的氢氧化钠溶液，在10％下提取30min，离心分离（3000r／min，15min），收集上清液，沉淀用于提取多糖。将上清液减压浓缩后，加乙醇至83％，使蛋白质沉淀下来。100g香菇粉制备的结果为：香菇蛋白得率为13．1％，其蛋白含量为47．5％；香菇多糖得率为5．6％，其多糖含量为69．5％。According to the difference of dissolvability between protein and polysaccharide in Lentinus edodes, protein was isolated firstly, and the residue was used to extract polysaccharide.The methods of extraction and precipitation of protein from Lentinus edodes were studied.The results showed that the extraction and preparation methods of protein from Lentinus edodes were. adding 10- 20 times NaOH solution （pH10） into the powder of Lentinus edodes, extracting at 10℃ for 30 minutes, centrifuging at 3000r/min for 15 minutes, collecting the supernatant liquid and the precipitation being used to extract polysaccharide.After concentrating the supernatant liquid in vacuo,ethanol was added to the supernatant till 83% concentration to precipitate the protein.From 100g of Lentinus edodes powder, 13.1 % protein of Lentinus edodes can be obtained, whose protein content was 47.5%, and 5.6% polysaccharide of Lentinus edodes can be obtained,whose polysaccharide content was 69.5%.

关 键 词：香菇 蛋白 多糖 提取 

分 类 号：TS201.21[轻工技术与工程—食品科学]