萼状粒毛盘菌多糖提取及其生物活性  

Extraction and Biological Activities of Polysaccharides from Fermented Mycelia of Lachnum calyculiforme

作　　者：叶明[1] 彭伟[1] 房聪[1] 李世艳[1] 杨柳[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2009年第16期69-72,共4页Food Science

基　　金：安徽省自然科学基金资助项目(090413086)

摘　　要：对一株YM-262萼状粒毛盘菌多糖的提取工艺及其多糖的抗氧化性及抑菌作用进行研究。结果表明,最佳提取工艺条件为:提取温度80℃,提取时间4h,液料比70:1,此条件下多糖得率可达9.16%。该多糖具有一定的抗氧化能力,浓度为30mg/L时,对DPPH自由基的清除率达到18.88%;多糖浓度为40mg/L时对·OH的清除率可达到50.43%;多糖浓度为100mg/L时,对O2·的清除率达到85.53%。该多糖对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌、酿酒酵母均有一定的抑制作用,其最小抑菌浓度为15.61～62.5μg/ml。The fermented mycelia of Lachnum calyculiforme YM-262 were extracted with hot water to produce polysaccharides. The effects of temperature of hot water, extraction duration and liquid/material ratio （V/m） on yield of polysaccharides were investigated by one-factor-at-a-time method. Subsequently, the factors were optimized to attain the maximum yield of polysac- charides using a three-factor and three-level orthogonal array design leading to a set of 9 experiments. Furthermore, polysaccha- rides obtained by optimized extraction procedure were analyzed for their antioxidant activity and antimicrobial activity. The optimum conditions for extraction of polysaecharides were determined as follows： with a ratio of liquid to material of 70：1, extraction at 80 ℃ for 4 h, and a yield of polysaccharides of 9.16% was achieved by the optimized extraction procedure. The polysaccharides were found to possess considerable antioxidant activity--their scavenging rate to DPPH radicals was 18.88% at 30 mg/L, to hydroxyl radicals 50.43% at 40 mg/L, and to superoxide anion radicals 85.53% at 100 mg/L. The polysaccharides also displayed certain antimicrobial activities to Escherichia.coli, Bacillus subtilis, Staphylococcus aureus and Saccharomyces cerevisiae, with a minimum inhibitory concentration （MIC） in the range of 15.61 - 62.5 μ g/ml.

关 键 词：多糖 抗氧化性 抑菌作用 萼状粒毛盘菌 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]