内蒙古传统乳制品中产细菌素乳酸菌的筛选及鉴定  

Screening and identification of bacteriocin producing Lactic acid bacteria from inner mongolia traditional dairy products

作　　者：张彦斌[1] 方芳[1] 李莉[1] 杜晓华[1] 剧柠[1] 孟和毕力格[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018

出　　处：《中国乳品工业》2009年第8期9-12,共4页China Dairy Industry

基　　金：内蒙古农业大学博士基金(K31610)

摘　　要：通过纸片法初筛、牛津杯法复筛(排除有机酸、H2O2的干扰以及胰蛋白酶和胃蛋白酶处理)的方法,从分离自内蒙古传统乳制品的135株乳酸菌中筛选到1株具有广谱高效的产细菌素乳杆菌WH12-2-1,经生理生化学特性和16S rDNA序列同源性分析,将该菌株鉴定为戊糖乳杆菌。A bacteriocin producing strain WH12-2-1 was screened from several lactic acid bacteria which were isolated from traditional products in Inner Mongolia by scrip method and cylinder-dish method （eliminated the interference of organic acid and H202, treated with trypsin and pasain）. The supernatant of WH12-2-1 had strong bacteriostatic activity against several gram-positive bacteria and gram-negative bacteria. The strain was identified as Lactobacillus pentosus by physiological-biochemical testing and 16S rDNA gene sequence homology analysis.

关 键 词：戊糖乳杆菌 细菌素 抑菌活性 

分 类 号：Q93-331[生物学—微生物学]