嗜酸乳杆菌胞外多糖发酵条件的优化  

Optimization of Fermentation Conditions of Lactobacillus acidophilus for Extracellular Polysaccharide Production

作　　者：吕思伊[1] 刘齐[1] 黄行健[1] 卢琪[1] 周荧[1] 潘思轶[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2009年第19期255-258,共4页Food Science

基　　金：湖北省农业科技创新团队项目(2007-620)

摘　　要：采用Placket-Burman法从影响嗜酸乳杆菌胞外多糖产量的16个因素中筛选出3个主要因素,然后通过响应面法探讨最优工艺参数。实验结果表明:影响嗜酸乳杆菌胞外多糖产量的主要因素是吐温-80、磷酸氢二钾以及柠檬酸三胺,其最佳用量是吐温-800.5ml/L,磷酸氢二钾3.95g/L,柠檬酸三胺4.2g/L,在此条件下发酵的嗜酸乳杆菌胞外多糖产量达3.99g/L。Plackett-Burman method was adopted to screen main factors from 16 ones affecting Lactobacillus acidophilus extracel/ular polysaccharide yield. Based on a Box-Behnken central composite design involving 15 experiments of 3 variables at 3 levels, the interactive effects among the factors were analyzed by response surface methodology. Results showed that the most significant effects on the fermentation process due to concentrations of Tween 80, potassium dihydrogen phosphate and citric acid triamine in medium were observed; the optimal values of their concentrations were determined as follows： Tween 80 0.5 ml/L, potassium dihydrogen phosphate 3.95 g/L, citric acid triamine 4.2 g/L; a maximum extraeellular polysaccharide yielof 3.99 g/L was achieved at these optimal concentration levels.

关 键 词：嗜酸乳杆菌胞外多糖 响应面法 吐温-80 磷酸氢二钾 柠檬酸三胺 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]