啤酒废酵母中β-1,3-葡聚糖的提取工艺  

Extraction of β-1,3-glucan from beer waste yeast

作　　者：马森[1,2] 卢家炯[1,2] 林金梅[1,2] 赵迎春[1,2] 韦宇[1,2] 

机构地区：[1]广西大学糖业工程技术中心,南宁530004 [2]广西大学轻工与食品工程学院,南宁530004

出　　处：《生物加工过程》2009年第5期39-43,共5页Chinese Journal of Bioprocess Engineering

基　　金：广西重大科技成果引进与产业化示范资助项目(0630004)

摘　　要：研究采用酶-碱法从经超声波处理的废酵母残渣中提取β-1,3-葡聚糖的工艺,通过正交试验得出理想的酶处理工艺条件:酶添加量208 U/g,温度50℃、pH 6,酶解8 h,蛋白质去除率为62.82%,每L废酵母液中可回收0.348 g多肽、氨基酸的蛋白水解液;碱处理工艺条件:用30 mL质量分数为2%NaOH溶液在70℃处理酶解后的沉淀物5 h。所得β-1,3-葡聚糖纯度为90.50%,得率为11.00%,经紫外光谱、薄层层析和性质分析为高纯度的β-1,3-葡聚糖。The enzyme-alkali extracting method for the β-1,3-glucan from beer waste yeast treated by ultrasonic waves was studied. Papain enzyme of 208 U/g substrate was added in the beer yeast and incubated for 8 h at pH 6 and 50 ℃. Protein removal rate was 62.82%. Protein hydrolysate （0. 348 g/L） containing po lypeptides and amino acids was recycled. The precipitate was treated with 30 mL 2% NaOH and water-bathed 5 h at 70 ℃. The yield of r-1,3-glucan was 11.00% by purity of 90.50%.

关 键 词：提取 β-1 3-葡聚糖 啤酒废酵母 

分 类 号：TQ929.2[轻工技术与工程—发酵工程] Q538[生物学—生物化学]