双酶改性制备玉米皮水溶性膳食纤维的工艺研究  

Use of Xylanase and Cellulase for Preparation of Soluble Dietary Fiber from Corn Bran

作　　者：侯传伟[1] 魏书信[1] 王安建[1] 

机构地区：[1]河南省农业科学院农副产品加工研究所,河南郑州450002

出　　处：《食品科学》2009年第22期119-121,共3页Food Science

基　　金：河南省重点科技攻关项目(072102110006)

摘　　要：以玉米皮超声提取天然水溶性膳食纤维后的副产物——不溶性玉米皮渣为试材,应用木聚糖酶和纤维素酶组合酶解制备水溶性膳食纤维,采用单因素和正交试验组合研究确立一套由水不溶性膳食纤维改性制备水溶性膳食纤维制备工艺。结果表明,最佳工艺参数为纤维素酶添加量40mg/g底物、木聚糖酶添加量40mg/g底物、料液比1:14(g/ml)、酶解时间90min,水溶性膳食纤维得率为5.96%。The insoluble fiber in corn bran by-product after ultrasonic-assisted extraction of natural soluble dietary fiber was further hydrolyzed using xylanase and cellulase for preparation of soluble dietary fiber.By conducting single factor experiments and orthogonal tests,conditions for the enzymatic hydrolysis were optimized as follows：cellulase 40 mg/g substrate,xylanase 40 mg/g substrate,solid-to-liquid ratio 1：14,extraction time 90 minutes.Under such conditions,the yield of SDF was 5.96%.

关 键 词：不溶性玉米皮渣 木聚糖酶 纤维素酶 水溶性膳食纤维 

分 类 号：TS201.1[轻工技术与工程—食品科学]