产4-羟基-2,5-二甲基-3(2H)-呋喃酮酵母菌的分离、筛选及分子生物学鉴定  

Isolation,screening and molecular biological characterization of yeasts producing 4-hydroxy-2,5-dimethyl-3(2H)-furanone(HDMF)

作　　者：王鹏霄[1] 程林坤[1] 陈存社[1] 吕跃钢[1] 宋焕禄[1] 

机构地区：[1]北京工商大学化学与环境工程学院,北京100048

出　　处：《食品工业科技》2010年第2期163-166,共4页Science and Technology of Food Industry

基　　金：北京市高校人才强教计划学术创新人才项目资助(PHR200906110)

摘　　要：以菠萝、草莓等水果为分离源,分离、筛选利用D-果糖产香料HDMF酵母菌株,并对其高产菌株进行分子生物学鉴定。采用稀释涂布法分离酵母菌株,高效液相色谱法检测HDMF产量,26SrDNAD1/D2区序列分析及系统发育分析鉴定菌株。共分离得到46株酵母,5株可利用D-果糖产HDMF;产量较高的两株菌为:C5(6.84mg/L)、P3(10.96mg/L),分别约为已报道毕赤氏酵母属(Pichia capsulata)利用L-(+)-鼠李糖产HDMF的3倍和5倍;26S rDNA D1/D2区序列分析及系统发育分析结果显示,P3与Pichia caribbica(毕赤酵母属),C5与Hanseniaspora sp.(有孢汉逊酵母属)相似性均在99%以上,分子生物学法鉴定P3为Pichia caribbica,C5为Hanseniaspora sp.Yeasts having the abilities for producing 4-hydroxy-2,5- dimethyl-3 （2H）-furanone （HDMF） were screened from fruits such as pineapple and strawberry using D-fructose as carbon source.Spread plate method was used to separate yeasts strain from fruits, HPLC was used to determine the yield of HDMF by yeasts.26S rDNA D1/D2 area sequential analysis and phylogenetic analysis were used to indentify the strains. 46 Yeasts were obtained,of which 5 had the abilities of producing HDMF using D-fructose;2 strains had much higher yield. C5 （6.84rag/L） and P3（10.96mg/L） ,their yield were 3 and 5 times of the reported Pichia capsulata,which produced HDMF by using L-（ ＋ ）-rhamnose.The results of 26S rDNA DI/D2 area sequential analysis and phylogenetic analysis showed that the similarity of P3 with Pichia capsulata and C5 with Hanseniaspora sp were both over 99%. P3 was indentified as Pichia capsulata and C5 was Hanseniaspora sp by molecular biological identification.

关 键 词：酵母菌 微生物发酵 HDMF 26S RDNA 

分 类 号：TS201.3[轻工技术与工程—食品科学]