高效液相色谱法测定普洱茶中茶氨酸、EGCG及咖啡因  

Theanine,EGCG and caffeine determinated by HPLC

作　　者：刁飞燕[1] 董凤鸣[1] 王秋丽[1] 刘娜[1] 郭冬梅[1] 崔晞[1] 

机构地区：[1]山东大学公共卫生学院卫生检验研究所,济南250012

出　　处：《卫生研究》2010年第1期107-109,共3页Journal of Hygiene Research

摘　　要：目的建立了未衍生化高效液相色谱法(HPLC)测定普洱茶中茶氨酸含量,非梯度洗脱的HPLC分析方法测定表没食子儿茶素没食子酸酯(EGCG)及咖啡因含量。方法普洱茶中的茶氨酸在热水浴中以水提取,EGCG及咖啡因以70%的乙醇超声提取,采用C18色谱柱,HPLC-PDAD方法进行测定,流速为1.0ml/min。以乙腈-0.005mol/L十二烷基磺酸钠(0.1%磷酸调pH为6)(28:72)为流动相测定茶氨酸,以甲醇-5×10-5mol/LKH2PO4溶液(磷酸调pH为5)(20:80)为流动相测定EGCG及咖啡因。结果在2～400μg/ml浓度范围内,各组分峰面积与相应浓度呈良好线性关系,r>0.999。各组分的加标回收率在85%～110%之间,方法精密度相对标准偏差小于10%。结论该方法准确、稳定、分离效能好。Objective A high-performance liquid chromatography（HPLC） method was developed for the determination of theanine in puer tea without derivatization and HPLC for EGCG and caffeine with ingredient elution system was put forward.Methods The theanine in the tea was extracted with water.The EGCG and caffeine was extracted with water∶ethanol （3∶7） by ultrasonic oscillation.After centrifugation,the extract was analyzed by HPLC-PDAD with a C 18 column and at the flow rate 1ml/min.The theanine was determinated with 5mmol/L SDS-acetonitrile mobile phase system（72∶28）,and both EGCG and caffeine with 0.05mmol/L KH2PO4-methonal mobile phase system（80∶20）.Results The calibration of three ingredients was in good linearity.The recovery range is 85%-110%.The RSD is less than 10%.Conclusion The method is accurate and stable.

关 键 词：茶氨酸 EGCG 咖啡因 普洱茶 高效液相色谱法 食品检测 

分 类 号：TS207.3[轻工技术与工程—食品科学] TS272.7[轻工技术与工程—食品科学与工程]