β-葡萄糖苷酶基因工程菌发酵工艺优化  

β-Glucosidase Production under Optimized Fermentation Conditions of Genetically Engineered Bacterial Strain

作　　者：刘长江[1] 梁爽[1] 郑艳[1] 宗绪岩[1] 李长彪 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110161 [2]抚顺市农村经济委员会,辽宁抚顺113000

出　　处：《食品科学》2010年第11期173-177,共5页Food Science

基　　金：辽宁省科技厅科技攻关项目(2004205001)

摘　　要：以菌体生物量和酶比活力为参考指标,通过单因素和响应面分析法优化β-葡萄糖苷酶基因工程菌发酵条件,得到最优的发酵条件:温度37.9℃、pH6.6、接种量10%、转速200r/min、诱导时间10h,在此条件下发酵可以使β-葡萄糖苷酶酶活力达到147.8U/L。The fermentation conditions of genetically engineered bacterial strain for β-glucosidase production were optimized using one-factor-at-a-time method and response surface analysis based on central composite design.The optimal procedure for β-glucosidase production was based on 8 h fermentation under the conditions of pH 6.6,37.9 ℃,10% inoculation amount,200 r/min rotation speed followed by 10 h of induction with the addition of 0.5 mmol/L IPTG,which made the enzyme activity and of β-glucosidase reach up to 147.8 U/mL,respectively.

关 键 词：Β-葡萄糖苷酶 基因工程 发酵工艺 

分 类 号：Q784[生物学—分子生物学]