检测产志贺毒素大肠杆菌的菌落PCR方法  

A Colony PCR Assay for Detecting Shiga toxin-producing Escherichia coli

作　　者：李睿[1] 许芳[1] 张忠美[1] 宫本敬久[2] 朱廷恒[3] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]日本九州大学农学部生命科学与技术学院 [3]浙江工业大学生物与环境工程学院,浙江杭州310000

出　　处：《食品科学》2010年第16期258-260,共3页Food Science

基　　金：湖北省自然科学基金重点项目(2009CDA118)

摘　　要：针对产志贺毒素大肠杆菌的毒力基因stx设计特异性引物,并建立一种菌落PCR方法。菌落PCR模拟实验证实,该方法特异性强,能良好的扩增出O157的stx1和stx2基因,而普通大肠杆菌、蜡样芽孢杆菌、金黄色葡萄球菌则无PCR扩增产物。应用分子检测初筛、选择性培养、菌落PCR相结合的方法,检测实际食品样品,分离检测到一株携带stx1的产志贺毒素大肠杆菌。本实验建立的菌落PCR方法可应用于食品检验。Specific primers targeting for the stx gene of Shiga toxin-producing Escherichia coli （STEC） were designed, and a colony PCR assay method was then developed based on first screening and selective culture in this study. The assay method could specifically identify E. coli O157 carrying stx1 and stx2 genes, while normal E. coli, Bacillus cereus and Staphylococcus aureus could not produce any PCR products. A STEC strain carrying stx1 gene was detected using the assay method in commercial pork and beef products. The method developed in this study is applicable to food samples for the detection of STEC.

关 键 词：产志贺毒素大肠杆菌 食品检验 stx基因 菌落PCR 

分 类 号：R155.5[医药卫生—营养与食品卫生学]