离子交换固相萃取高效液相色谱联用法检测食品中的5-羟甲基糠醛  

Development of Ion-exchange Solid Phase Extraction and High Performance Liquid Chromatography for the Determination of 5-Hydroxymethyl-furfural in Food

作　　者：张燕[1] 郭天鑫[1] 于姣[1] 丰帆[1] 王硕[1] 

机构地区：[1]食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品科学》2010年第18期212-215,共4页Food Science

基　　金："十一五"国家科技支撑计划项目(2008BAD91B04)

摘　　要：为提高分析方法的准确性,优化建立一种定量检测各类食品中5-羟甲基糠醛含量的高效液相色谱方法。采用离子交换固相萃取柱代替传统的C18固相萃取柱进行前处理,与高效液相色谱联用,提高了方法的回收率和精密度。在0.01～12mg/L质量浓度范围内,5-羟甲基糠醛质量浓度和色谱峰面积线性关系好(R2=0.9998),方法检出限(RSN=3)为3.42μg/L。在0.1、0.5、1mg/kg3种质量浓度添加水平内,5-羟甲基糠醛的添加回收率为85%～103%,RSD为0.31%～3.83%。采用该方法对13种样品进行测定,结果表明该方法能够用于食品中5-羟甲基糠醛含量测定。An ion exchange solid phase extraction coupled with high performance liquid chromatographic method was presented and optimized in order to improve its sensitivity and accuracy.In this method,ion exchange solid-phase extraction column was used for pretreatment instead of conventionally used C18 solid-phase extraction column so that method recovery and accuracy could be both improved.This method exhibited a good linear relationship over the 5-hydroxymethyl-furfural（5-HMF） concentration range of 0.01 to 12 mg/L（R2 =0.9998）.The detection limit of this method was 3.42 μg/L.The recovery rates for 5-HMF spiked at levels of 0.1,0.5 mg/L and 1 mg/L were between 85% and 103% with a relative standard deviation varying from 0.31% to 3.83%.Determination of 5-HMF in 13 real samples was performed using this method,and the results demonstrate that this method is applicable to determine 5-HMF in food.

关 键 词：5-羟甲基糠醛 离子交换固相萃取 高效液相色谱 食品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]