马铃薯淀粉制备磷酸寡糖的研究  

Preparation of Phosphoryl Oligosaccharides from Potato Starch

作　　者：杨文军[1] 刘霞[1] 杨丽[1] 朱培蕾[2] 程莉莉[1] 杜先锋[1] 

机构地区：[1]安徽农业大学食品科学工程系,合肥230036 [2]安徽省农业科学院园艺研究所,合肥230031

出　　处：《中国粮油学报》2010年第11期52-56,共5页Journal of the Chinese Cereals and Oils Association

基　　金：863计划(2006AA10Z340)

摘　　要：以马铃薯淀粉为原料,采用全酶法并借以喷射液化工艺制备低DE值的麦芽低聚糖浆,采用高效阴离子交换色谱法测定所制备样品的糖分组成,离子树脂交换法从中分离制备磷酸寡糖,最后,采用薄层层析法和红外光谱法对其进行定性分析。结果表明:制备液化DE值16%,糖化DE值35%的糖液,其中,主要成分为麦芽二糖至麦芽七糖,以二糖至五糖居多。201×4强碱性苯乙烯系阴离子交换树脂分离效果好,分离条件为:洗脱液为0.4 mol/L NaCl,洗脱速度为1.4 mL/min。经红外光谱定性分析,结果显示样品为结合有磷酸基团的糖类物质。Malt-oligosaccharides with low dextrose equivalent（DE） was prepared from potato starch by using the fully enzymatic method and steam ejection liquefaction.Sample composition was detected using high performance anion-exchange chromatography,and phosphoryl oligosaccharides（POs） were separated from malt-oligosaccharides with the ion-exchange method.Finally,the POs were analyzed by thin-layer chromatography and infrared spectrum.Results： The DE values of the prepared liquefied and saccharified malt-oligosaccharides are 16% and 35%,respectively,and a majority of the product is malt-oligosaccharides of disaccharide to pentose.The optimal conditions to separate POs are using 201×4 ion-exchange resin for separation,and eluting the column with 0.4 mol/L NaCl at a speed of 1.4 mL/min.IR analysis indicates the product is phosphate groups bound saccharides.

关 键 词：马铃薯淀粉 磷酸寡糖 分离 定性分析 

分 类 号：TS245.9[轻工技术与工程—制糖工程]