微生物多糖韦兰胶生产工艺优化  

Fermentation Processing of Microbial Polysaccharide Welan Gum

作　　者：赵燕[1,2] 陈芳[1,2] 李建科[1,2] 廖斌[1,2] 涂勇刚[3] 

机构地区：[1]南昌大学生物质转化教育部工程研究中心,江西南昌330047 [2]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [3]江西农业大学食品科学与工程学院,江西南昌330045

出　　处：《食品科学》2010年第23期219-223,共5页Food Science

基　　金：食品科学与技术国家重点实验室自由探索课题(SKLF-TS-200822)

摘　　要：探讨韦兰胶的生产条件,主要包括生产菌株、发酵培养基及发酵工艺条件三方面。通过绘制菌体生长曲线,了解此菌种的生长情况,初步确定二级种子的培养时间。通过单因素试验及正交试验,得出韦兰胶的最佳发酵培养基配方为:蔗糖40g/L、酵母膏3g/L、K2HPO4·7H2O5g/L、MgSO4·7H2O2g/L、FeSO4·7H2O1mg/L、CaCl20.5g/L;此条件下,韦兰胶产率由7.31g/L上升到17.23g/L。最佳发酵工艺条件为:接种龄18h、接种量0.5%、装液量40mL(250mL摇瓶)、初始pH7.0、摇床转速220r/min、培养温度30℃、培养时间72h,在此条件下,韦兰胶产率达20.64g/L。Optimal preparation conditions such as strain, fermentation medium and fermentation processing parameters were explored. The growth of strain and second stage of seed were achieved by the growth curve of ATCC31555 in medium. The optimal fermentation medium for preparing Welan gum were explored by single factor and orthogonal experiments to be 40 g/L sucrose, 3 g/L yeast extract, 5 g/L K2HPO4·7H2O, 2 g/L MgSO4·7H2O, 1 mg/L FeSO4·7H2O and 0.5 g/L CaCl2. The yield of Welan gum was increased from 7.31 to 17.23 g/L by using the improved medium. The optimal fermentation conditions were the seed age of 18 hours, inoculation amount of 0.5% （V/V）, medium volume of 40 mL in 250 mL flask, pH 7.0, shaking speed of 220 r/min, fermentation temperature of 30 ℃ and fermentation time of 72 h. Under the optimal fermentation conditions, the yield of Welan gum was up to 20.64 g/L.

关 键 词：微生物多糖 韦兰胶 发酵 

分 类 号：TS201.3[轻工技术与工程—食品科学]