贻贝中ACE抑制活性肽的酶解制备及表征  

Preparation and Characterization of Mytilus Edulis Protein Hydrolysates with ACE Inhibitory Activity

作　　者：张艳萍[1,2] 戴志远[1] 张虹[1] 

机构地区：[1]浙江工商大学食品与生物工程学院,杭州310035 [2]浙江科技学院生物与化学工程学院,杭州310012

出　　处：《中国食品学报》2011年第1期51-59,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省水产品加工创新团队项目(2009R50031)

摘　　要：以贻贝为原料,制备具有血管紧张素Ⅰ转换酶(ACE)抑制作用的活性肽。采用水解度DH、蛋白质回收率及ACE抑制活性为指标,筛选水解贻贝粗蛋白的最适酶种;通过响应面分析(RSM)对水解贻贝粗蛋白的工艺条件进行优化;探讨水解度(DH)与ACE抑制活性的关系;利用HPLC方法分析活性肽的相对分子质量分布。试验结果表明碱性蛋白酶Alcalase 2.4L是水解贻贝粗蛋白的最适酶种;碱性蛋白酶在[E]/[S]1.64、pH9.12、温度57℃水解条件下,酶解物的ACE抑制作用最强,其DH26.41%,IC5034.64g/mL。就贻贝粗蛋白的水解而言,控制水解度为25%～30%为宜。碱性蛋白酶水解贻贝粗蛋白所得活性肽,主要是以相对分子质量较小的短肽组成,其中,相对分子质量在1000Da以内的占90.42%。本研究结果表明以贻贝为原料,可以开发具有显著ACE抑制活性的降血压肽。Mytilus edulis protein was hydrolyzed using six different proteases.The results showed that Alcalase 2.4L have the best hydrolyzing capacity,the higher protein recovery and the stronger ACE（Angiotensin-Ⅰ-Coverting Enzyme） inhibitory activity.So,Alcalase was chosen to produce Mytilus edulis protein hydrolysates with ACE inhibitory activity.Response surface methodology（RSM） was applied to optimize the hydrolysis conditions using Alcalase.The optimum values for enzyme/substrate ratio,pH and temperature were found to be 1.64%,9.12 and 57 ℃ respectively.Under those conditions,the DH was 26.41% and IC50 was 34.64 g/mL.Then,the relative molecular weight of hydrolysates was identified by HPLC.The results showed that Mytilus edulis protein hydrolysates were made up of short peptide with low molecular weight.The short peptides below 1 000 Da take 90.2%.Using Mytilus edulis as raw material,the depress on peptide with obviously ACE inhibitory activity can be prepared.

关 键 词：贻贝 酶解 ACE抑制 活性肽 

分 类 号：TQ464.7[化学工程—制药化工]