降胆固醇乳酸菌鉴定及其在体外模拟胃肠环境中抗性研究  

Identification of a cholesterol reducing lactic acid bacteria and its tolerance in in vitro gastroenteric environment

作　　者：赖文[1] 刘书亮[1] 张倩颖[1] 姜娟[1] 

机构地区：[1]四川农业大学食品学院,四川雅安625014

出　　处：《中国酿造》2011年第3期90-93,共4页China Brewing

基　　金：四川省科技厅项目(09ZC1270-4)

摘　　要：一株源于四川泡菜的具有降胆固醇能力的乳酸菌p5经形态学、生理生化特征和16SrDNA序列分析，鉴定为植物乳杆菌（Lac—tobacillus plantarump5）。在体外模拟胃肠环境中的抗性研究结果表明，p5具有较强耐酸能力，PH值为2．0时，2h活菌数保持在10^6cfu／mL；pH值为2．5时，4h内活菌数仍可保持在106cfu／mL，oH值为3．0-3．5时，p56h内活菌数可保持在106cfu/mL；p5具有耐高浓度胆汁盐的能力，在n1％胆汁盐MRS中，p5表现出生长趋势，在0．2%-0．5％胆汁盐MRS中，p5活菌数下降急速，但6h后活菌数能保持在10^5cfu/mL；p5对肠炎沙门氏菌、大肠杆菌、铜绿假单胞杆菌、金黄色葡萄球菌等肠道致病菌有明显抑制作用。p5可望作为食品工业用菌种的资源。The cholesterol reducing lactic acid bacteria strain p5 isolated fi＇om Sichuan pickle was identified as Lactobacillus plantamm p5 according to morphological, physiological and biochemical characteristics and 16S rDNA sequences analysis. Results of tolerance in in vitro gastroenteric environments indicated that the strain p5 is acid tolerance, which could keep viable cell counts at 106cfu/ml under the conditions ofpH value 2.0 for 2h, pH value 2.5 for 4h and pH value 3.0-3.5 for 6h. The strain is also bile tolerant. It grows well in MRS had 0.1% bile salts. When concentrations of bile salts at 0.2%-0.5%, the viable cell counts of p5 reduced rapidly reduced to 10^5cfu/ml after 6h inoculation. The strain had inhibition activities to pathogenic Salmonella enteritidis, Escherichia coli, Seudoraonas acmginosa and Staphylococcus aureus. L. plantarum p5 could be used as food industry strain resource.

关 键 词：乳酸菌 植物乳杆菌 抗性 抑菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]