顶空固相微萃取-气质联用快速测定大曲中的挥发性风味成分  

Rapid Analysis of Volatile Compounds in Daqu by Headspace Solid-phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

作　　者：张春林[1] 敖宗华[2] 炊伟强[1] 沈才洪[2] 陶文沂[1] 张宿义[2] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]泸州老窖股份有限公司,四川泸州646000

出　　处：《食品科学》2011年第10期137-140,共4页Food Science

基　　金：四川省科技支撑计划项目(2010SZ0032);酿酒生物技术及应用四川省重点实验室2008年度开放课题(NJ2009-01)

摘　　要：为探讨大曲中的挥发性风味成分,采用顶空固相微萃取-气相色谱-质谱法(HS-SPME-GC-MS)分析泸州老窖中高温大曲的挥发性成分,考察萃取头、萃取时间、萃取温度对大曲样品中挥发性风味物质萃取的影响。结果表明:采用50/30μm碳分子筛/聚二乙烯基苯/聚二甲基硅氧烷(DVB/CAR on PDMS)纤维头在50℃条件下对大曲样品顶空吸附30min,萃取效果最好;大曲样品的分析表明,其挥发性风味物质中含量较高的是醇类、酯类和酮类化合物,另外还含有一些吡嗪类化合物。该方法对大曲中常见挥发性风味成分的测定有良好的重复性和回收率,方便快速。In order to analyze the composition of volatile compounds in the Luzhou Laojiao Daqu by headspace solid phase micro-extraction coupled with gas chromatography mass spectrometry detection（HS-SPME-GC-MS）,the effects of SPME fiber type,adsorption time,and extraction temperature on volatile extraction were investigated.Using a 50/30 μm DVB/CAR on PDMS fiber for 30 min headspace adsorption at 50 ℃ provided optimum extraction for the Daqu.Then the fiber was introduced into the injection port of the GC-MS system（at 250 ℃ for 5 min）.Fifty-five compounds were isolated and identified,among which alcohols,esters,and ketones were the prominent ones.In addition,some pyrazines were also found.In conclusion,the method is simple,fast and accurate with high reproducibility and sensitivity.

关 键 词：顶空固相微萃取法 气相色谱-质谱联用 泸州老窖大曲 挥发性风味化合物 

分 类 号：TS261.2[轻工技术与工程—发酵工程]