苹果渣发酵饲料活性物质含量及影响因素研究  

The influence factors on contents of active substance in apple pomace fermentation feed

作　　者：任雅萍[1] 薛泉宏[2] 来航线[2] 

机构地区：[1]西北农林科技大学生命学院 [2]西北农林科技大学资源环境学院

出　　处：《饲料工业》2011年第12期35-39,共5页Feed Industry

基　　金："十一五"国家科技支撑计划项目[2007BAD89B16];杨凌农业高新技术产业示范区农业推广专项[YLTG2005-8]

摘　　要：研究供试发酵剂、原料组成及灭菌方式对苹果渣发酵单细胞蛋白饲料中游离氨基酸、活性肽及水溶性蛋白含量的影响,为果渣发酵饲料品质研究提供新的科学依据。以未发酵纯果渣原料作为对照组,设发酵剂、灭菌方式及原料组成3个因素,采用固态发酵及比色法对发酵产物进行品质分析。结果表明:接菌、灭菌及添加油渣处理后发酵产物游离氨基酸含量为10.19～17.96 g/kg,较对照组增加87.7%～230.8%;生物活性肽含量为0.71～0.96 g/kg,较对照组增加255.0%～380.0%;水溶性蛋白质含量为7.89～14.87 g/kg,较对照组增加279.3%～614.9%。采用混菌发酵,在原料中添加油渣和氮素及灭菌处理对提高苹果渣发酵饲料游离氨基酸、生物活性肽以及水溶性蛋白质含量有显著作用(P<0.05)。In this experiment,solid state fermentation and colorimetric spectroscopy were used in order to acquire an optimal combination of the texted starter cultures,ingredients and sterilization manners,which would affect the total free amino acid,bioactive peptides and water-soluble protein contents of apple pomace fermentation feed.Pure apple pomace without fermentation was used as the control,3 factors of texted starter cultures,ingredients and sterilization manners were assigned containing 24 treatments with three replicates.The results showed that,the contents of the total free amino acid,bioactive peptides and water-soluble protein of the inoculum samples reached 10.19～17.96,0.64～0.96 and 5.94～14.87 g/kg,respectively,which were enhanced by 87.7%～230.8%,178.3%～317.4%,185.6%～614.9%,separately,compared with blank samples.The total free amino acid,bioactive peptides and water-soluble protein contents of apple pomace fermentation feed have been obviously improved by the sterilization,mixed microorganisms fermentation treament,combined with addition of oil foot and nitrogen in raw material.

关 键 词：苹果渣 发酵饲料 游离氨基酸 生物活性肽 水溶性蛋白 

分 类 号：TQ92[轻工技术与工程—发酵工程]