抗酒精化学损伤的茶氨酸复合配方筛选与体外抗氧化活性研究  

Screening of Theanine Complex for Inhibition of Alcohol Chemical Injury and its In Vitro Antioxidant Activity

作　　者：李大伟[1] 张士康[1] 朱跃进[1] 施海根[1] 

机构地区：[1]中华全国供销合作总社杭州茶叶研究院,浙江杭州310016

出　　处：《中国茶叶加工》2011年第3期37-41,共5页China Tea Processing

摘　　要：通过流动注射化学发光法筛选以茶氨酸为主要成分的强抗氧化性配方,研制以茶氨酸、γ-氨基丁酸等茶叶特征成分为主要配方的复合氨基酸颗粒剂(以下简称功能修饰因子)。采用不同溶解载体溶解不同浓度的功能修饰因子,通过对.OH、DPPH.、H2O2清除能力和过氧化脂质抑制作用的测定,研究功能修饰因子的抗氧化性能。结果表明,以水、42%乙醇、42%茶酒作为溶解载体溶解的功能修饰因子,均具有较好的抗氧化性能,其中功能修饰因子的42%茶酒溶液在较低浓度就显示出很好的清除效果,且浓度与清除能力呈现明显的量效关系,表明茶酒中以儿茶素为主体的茶叶功效成分与功能修饰因子具有较强的协同增效作用。当浓度为4g/mL、1g/mL、2.5g/mL、5g/mL时,对.OH、DPPH.、H2O2、过氧化脂质的清除率最高,分别为61.8%、61.92%、51.05%、85.93%。Flow-injection chemiluminescence was used to determine the antioxidant activity of product mainly composed of theanine.Complex was prepared by theanine and γ-amino butyrate as main antioxidants（Hereinafter referred to as functional modifier）.The antioxidant activities,including the ·OH radical,DPPH· radical,H2O2 scavenging activities and inhibition of Lipid peroxide,of the complex solutions with differed amount in different solvents were determined.The results showed that water,42% ethanol,42% tea wine as a function of solvent modifier,has better oxidation resistance,in which the function modifier solution in the lower 42% of tea wine shows good concentration on the removal of effect,and the concentration and scavenging obviously showed dose-response relationship,indicating that the catechins in tea and wine as the main effect of composition and function of the tea has a strong modifying factor synergies.When the concentrations were 4 g/mL,1g/mL,2.5g/mL,5g/mL,the·OH,DPPH·,H2O2,lipid peroxidation highest clearance rates were 61.8%,61.92%,51.05%,85.93%.

关 键 词：茶氨酸 茶特征成分 功能修饰因子 抗氧化活性 自由基 

分 类 号：TS201.2[轻工技术与工程—食品科学]