巨大芽孢杆菌发酵降解亚硝酸盐动力学研究  

Fermentation characteristic of nitrite reduction by Bacillus megaterium MPF-906

作　　者：张莹[1] 刘萍[1] 孙君社[1] 罗岩[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国酿造》2011年第10期24-28,共5页China Brewing

基　　金：国家863计划课题(2008AA10Z306);国家自然基金(20876164)

摘　　要：研究了在不同初始亚硝酸钠和葡萄糖浓度条件下,巨大芽孢杆菌B.megaterium MPF-906细胞生长和降解亚硝酸盐的发酵动力学。结果发现,初始亚硝酸盐浓度对细胞生长有重要影响,初始亚硝酸盐浓度为2mmol/L的环境最利于细胞增殖,此时亚硝酸盐的消耗速率也最大,发酵液中亚硝酸盐消耗曲线和细胞生长曲线表现出互为对应的关系;低浓度诱导物亚硝酸钠的环境也更有利于细胞生产亚硝酸还原酶(NiR)。初始葡萄糖浓度对细胞生长也有重要影响,初始葡萄糖浓度为2.0%的环境最利于细胞增殖,此时细胞生成速率最大,初始较高的葡萄糖浓度时,亚硝酸盐的降解速率减小,但亚硝酸盐降解率仍可在14h内达到100%;初始葡萄糖浓度为2.0%时,糖耗速率最快;较高浓度葡萄糖的环境也有利于细胞生产NiR。The effects of different initial concentrations of sodium nitrite and glucose on the fermentation of nitrite reductase （NiR） were investigated. Results showed that sodium nitrite concentration had a significant effect on cell growth, with 2mmol/L NaNO2 at initial time, the highest cell growth rate and consumption rate of sodium nitrite were obtained. The corresponding relationship was observed between the curve of nitrite degradation and the curve of cell growth. With low initial sodium nitrite concentration, the NiR activity was stimulated. Glucose concentration also had a significant effect on cell growth. With 2.0% glucose at initial time, the highest cell growth rate and glucose consumption rate were observed, and the consumption of nitrite could reach 100% in 14h. High initial glucose concentration could stimulate the NiR activity.

关 键 词：巨大芽孢杆菌 亚硝酸还原酶 亚硝酸盐 发酵动力学 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]