Enzyme-linked Immunosorbent Assay for Detection of Anti-idiotype Antibodies to Antibodies to Ligand of Nicotinic Acetylcholine Receptor in Sera of Patients with Myasthenia Gravis  

作　　者：黄德仁 涂来慧 张仁琴 周广智 沈茜 

机构地区：[1]Department of Neurology,Changhai Hospital,Second Military Medical University,Shanghai 200433 [2]Clinical Laboratory,Changhai Hospital

出　　处：《Journal of Medical Colleges of PLA(China)》1990年第3期237-242,共6页中国人民解放军军医大学学报（英文版）

摘　　要：Anti-bungarotoxin anti-serum,which has the internal image of nicotinicacetylcholine receptor,was used as a tool to measure anti-idiotypic antibodies toantibodies to Iigand of nicotinic acctylcholine receptor in scra from 81 patients withmyasthenia gravis.Enzyme-linked immunosorbcnt assay was adopted.Thc positive ratewas 46.9%(38/81).The specific cross inhibitory test with nicotinic acetylcholinereceptor was positive.Anti-idiotype antibodies to antibodies to ligand of nicotinicacetylcholine receptor in sera of different types of myasthenia gravis patients classified ac-cording to modified Osserman’s standard and myasthenia gravis patients with or withoutthymoma were comparcd in this study and the role of anti-idiotype antibodies toantibodies to Iigand of nicotinic acctylcholinc receptor in the immunity of myasthcniagravis and the possibility of thcrapeutic use of anti-idiotype antibodies arc discussed.Anti-bungarotoxin anti-serum,which has the internal image of nicotinic acetylcholine receptor,was used as a tool to measure anti-idiotypic antibodies to antibodies to Iigand of nicotinic acctylcholine receptor in scra from 81 patients with myasthenia gravis.Enzyme-linked immunosorbcnt assay was adopted.Thc positive rate was 46.9%(38/81).The specific cross inhibitory test with nicotinic acetylcholine receptor was positive.Anti-idiotype antibodies to antibodies to ligand of nicotinic acetylcholine receptor in sera of different types of myasthenia gravis patients classified ac- cording to modified Osserman's standard and myasthenia gravis patients with or without thymoma were comparcd in this study and the role of anti-idiotype antibodies to antibodies to Iigand of nicotinic acctylcholinc receptor in the immunity of myasthcnia gravis and the possibility of thcrapeutic use of anti-idiotype antibodies arc discussed.

关 键 词：MYASTHENIA gravis enzyme-linked immunosorbent assay NICOTINIC acetylcholine receptor LIGAND antibungarotoxin ANTISERUM ANTI-IDIOTYPE ANTIBODIES 

分 类 号：R[医药卫生]