无患子提取物对Fusarium sp.发酵产脂肪酶的影响及其双水相萃取分离  

Effect of Soapberry Extraction on Lipase Activity of Fusarium sp. and Lipase Extraction by Aqueous Two-Phase Systems

作　　者：张海蓉[1] 蔡宇杰[1] 廖祥儒[1] 张峰[1] 张大兵[2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江苏汉邦科技有限公司,江苏淮安223001

出　　处：《食品与生物技术学报》2011年第6期928-933,共6页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2010AA101501)

摘　　要：研究了表面活性剂对Fusarium sp.的发酵产脂肪酶的影响。结果表明,添加无患子提取物的酶活为最高,说明无患子提取物是最佳的的表面活性剂,并且确定提取无患子提取物的液固比为6∶1(mL∶g)时酶活达到最高。粗酶液经(NH4)2S04沉淀后进行双水相萃取分离,结果表明,采用质量浓度为30g/dL的PEG(M 4000)和质量浓度为20g/dL的NaH2PO4时纯化系数F和萃取率Sf达到最高;经过双水相萃取后,该脂肪酶的纯化倍数可以达到7.92,回收率为48.30%。Effect of surfactant on lipase production of Fusarium sp.was investigated in this manuscript.The results showed that lipase activity was achieved at the maximum value in adding soapberry extraction,which indicated that soapberry extraction is the best surfactant,and lipase activity was the highest when the liquid-to-solid ratio is 6∶1（mL∶g）.Lipase extraction by Aqueous Two-phase System following the precipitation of the crude enzymes by ammonium sulfate was also studied.The results indicated that the highest of decontamination factor F and extraction rate Sf achieved when the conditions of extraction showed as follows：the molecular weight of PEG was 4000,the concentration of PEG（M 4000）was 30 g/dL,and the concentration of NaH2PO4 was 20 g/dL.At last,the lipase was purified 7.92 folds and the recovery was 48.30% by Aqueous Two-phase Systems.

关 键 词：脂肪酶 发酵 无患子 酶活 双水相 

分 类 号：TQ925[轻工技术与工程—发酵工程]