新疆哈萨克族传统发酵酸马奶中酵母菌的分离鉴定  

Isolation and Identification of Yeasts from Koumiss as a Traditional Kazakh Fermented Drink in Xinjiang

作　　者：李静[1] 贾佳佳[1] 杨艳[1] 田燕[1] 武运[1] 

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《食品科学》2012年第5期203-207,共5页Food Science

基　　金：国家公益性行业(农业)科研专项(200803033-A09011);新疆维吾尔自治区科技支疆项目(201191238);新疆维吾尔自治区重大专项(201130101-4);新疆农业大学紧缺人才专业大学生创新项目

摘　　要：从新疆南山、水西沟、伊犁昭苏采集的哈萨克族传统发酵酸马奶中共分离得到酵母菌34株。采用传统形态学、生理生化特性方法对酵母菌进行鉴定,鉴定结果为Schizosaccharomyces 1株、Sehizoblastosporion 1株、Torulopsis 1株、Saccharomycodes 1株、Kloechera 9株、Kluveromyces 5株、Dekker 8株、Trichosporon 3株、Hansenula 2株、Brettanomyces 3株。利用5.8S rDNA序列同源性分析和系统发育树对菌株12号进行分子生物学鉴定,鉴定结果为:菌株12与标准菌株HQ396523.1同源性达到96%,为Kluveromyces的Kluveromyces marxianus,与传统鉴定结果一致,表明5.8S rDNA序列分析在酵母菌鉴定上的准确性。In this study, 34 stains of yeasts were isolated from koumiss （a traditional fermented Kazakh drink in Xinjiang） sampled from Nanshan, Shnixigou and Zhaosu. According to the colonial morphology and biological properties, the strains were identified to consist of I stain of Schizosaccharomyces, I stain of Sehizoblastosporion, I stain of Torulopsis, 1 stain of Saccharomycodes, 9 stains of Kloechera, 5 stains of Kluyveromyces, 8 stains of Dekker, 3 stains of Trichosporon, 2 stains of Hansenula, and 3 stains of Brettanomyces. Strain 12 was further identified by 5.8S rDNA sequencing and phylogenetic tree analysis. The homology between the strain and Kluyveromyces marxianus was 96%. This was consistent with the results of identification based on morphological and biochemical properties, showing that yeast identification based on 5.8S rDNA sequences is accurate.

关 键 词：酸马奶 酵母菌 分离鉴定 分子生物学 

分 类 号：TS201.3[轻工技术与工程—食品科学]