稻草粉基混合菌发酵产纤维素酶研究  

Cellulases produced by mixed strains fermentation in straw powder

作　　者：段学辉[1] 胡明明[1] 熊福星[1] 吴量[1] 陈加利[1] 王娟[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,生命科学与食品工程学院,江西南昌330047

出　　处：《食品工业科技》2012年第16期224-227,250,共5页Science and Technology of Food Industry

摘　　要：以稻草粉为主要原料,对白腐菌(White-rot fungi)NS75和黑曲霉(Aspergillus niger)NS83进行固态混合发酵产纤维素酶进行研究。混合菌固态发酵与单菌固态发酵实验结果比较表明,混合菌发酵产生的纤维素酶总体酶活明显高于单菌种发酵,其β-葡萄糖苷酶(β-G)酶活较白腐菌NS75单菌发酵提高了120.9%;葡聚糖内切酶(CMC)酶活比黑曲霉NS83单菌发酵提高了140.8%。单因素实验和正交实验结果表明,当稻草粉麸皮质量比为9∶1,料水比为1∶2,白腐菌NS75与黑曲霉NS83的接种比例为1∶1(v∶v)时,培养第3d开始搅拌,每天搅拌一次,于30℃,培养5d,稻草粉基混合菌发酵产纤维素酶中CMC酶活达到16650U/g,β-G酶活为16108U/g、滤纸酶活(FPA)酶活为3164U/g。Straw powder was used as the raw material,the solid fermentation conditions of cellulases production by mixed strains of White-rot fungi NS75 and Aspergillus niger NS83 had been investigated. The results of comparative experiments between single strain solid-state fermentation and mixed strains solid-state fermentation showed that,the cellulose enzyme activity produced by mixed strains had increased obviously than that produced in single strain solid-state fermentation,in which,the 15-G enzyme activity had increased 120.9% than that produced by White-rot fungi NS75 single fermentation,and the CMC enzyme activity had increased 140.8% than that produce by Aspergillus niger NS83 single fermentation. According to the single factor and orthogonal experiment results the optimum fermentation conditions were determined as follows： ratio between straw powder and bran powder 9：1 ,feed-water ratio 1：2,inoculation volume ratio of White-rot fungi NS75 and Aspergillus niger NS83 1：1 （v：v）,start stirring on the third day and once a day,30℃ culture 5d. Under this conditions,the enzyme activity of CMC enzymeJS-G enzyme,FPA enzyme produced had reached respectively 16650,16108 and 3164U/g.

关 键 词：混合菌发酵 固态发酵 CMC酶 β-G酶 稻草粉 

分 类 号：TS201.25[轻工技术与工程—食品科学]