饮料和糖果中32种水溶性色素的HPLC筛选性检测  

Screening Determination of 32 Kinds of Water-soluble Pigments in Beverages and Candy by HPLC

作　　者：顾宇翔[1] 葛宇[1] 印杰[1] 虞成华[1] 陆志芸[1] 刘孟鲲[1] 马晓飞[1] 

机构地区：[1]上海市质量监督检验技术研究院/国家食品质量监督检验中心,上海200233

出　　处：《食品工业》2012年第8期142-145,共4页The Food Industry

基　　金：国家质量监督检验检疫总局科研项目项目编号2009QK106

摘　　要：本研究建立了饮料和糖果中32种水溶性色素的HPLC筛选性检测方法。样品经水提取,聚酰胺粉末吸附,氨水/甲醇洗脱,XDB C18柱分析(250 mm×4.6mm,5μm),乙酸铵—甲醇—乙腈梯度洗脱检测。32种色素分为2组,检测波长分别选取450 nm,490 nm,520 nm和620 nm。此方法重现性相对标准偏差(n=6)为0.51%～7.8%,回收率70.0%～117.2%,检出限0.03 mg/kg～0.6 mg/kg,适用于饮料和糖果中多种水溶性色素的筛选性检测。Developed a HPLC screening method to determine 32 kinds of water-soluble pigments in beverages and candy.Samples were extracted by water,through polyamide absorption and ammonia/methanol desorption,finally analyzed by XDB C18 column（250 mm ×4.6mm,5 μm）.The mobile phase of gradient elution was： ammonium acetate-methanol-acetonitrile.32 kinds of pigments were divided into 2 groups,detection wavelength was 450 nm,490 nm,520 nm and 620 nm,respectively.The R.S.D.of samples reproducibility（n=6） was 0.51%~7.8%;the recovery of the method was in the range of 70.0%~117.2%;the limition of detection was 0.03mg/kg^0.6 mg/kg.The established method was suitable for screening determination of several water-soluble pigments in beverages and candy simultaneously.

关 键 词：HPLC 饮料 糖果 色素 筛选检测 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]