酶解及二次发酵豆粕生产富肽蛋白的研究  

Peptide-rich protein production by enzymolysis and secondary fermentation of soybean meal

作　　者：黄思敏[1] 刘军[1] 张锦盛[1] 孙东伟[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000

出　　处：《粮食与饲料工业》2012年第8期43-46,共4页Cereal & Feed Industry

摘　　要：利用黑曲霉和米曲霉混菌一次固态发酵所得豆粕,通过酶解及二次发酵生产富肽产品,研究酶解及二次发酵条件对生产富肽蛋白的影响。结果表明,一次发酵豆粕补3倍水磨浆、接种1%乳酸菌、活性干酵母1∶1混合菌种,木瓜蛋白酶0.3%,37℃发酵12h,再55℃酶解36h所得富肽蛋白小肽质量分数达到75.40%。Using soybean meal by solid-state fermentation with mixed strains of Aspergillus and Aspergillus oryzae,peptide-rich protein was produced by enzymolysis and secondary fermentation. The production condition of peptide-rieh protein was studied. The results Showed that：water in refining process added three times the soybean meal for primary fermentation,inocu-lated with 1% mixed strains（lactic acid bacteria ： active dry yeast= 1 ： 1）, then adding papain 0.3%, fermentation at 37℃ for 12 h,and enzymolysis at 55℃ for 36 h,the rich peptide protein content was up to 75.40%.

关 键 词：豆粕 酶解 二次发酵 富肽蛋白 氮溶指数 

分 类 号：S816[农业科学—饲料科学]