草鱼肽的体外生理功能  

in vitro Physiological Functions of Bioactive Peptides from Grass Carp Meat

作　　者：林利美[1] 陈季旺[1] 吴永宁 宫智勇[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023 [2]国家食品安全风险评估中心,北京100021

出　　处：《食品科学》2012年第15期34-37,共4页Food Science

基　　金：国家"863"计划项目(2010AA023003);武汉工业学院校研究生创新基金项目(2011cx013)

摘　　要：以草鱼为原料制取生理活性肽,采用高效液相色谱法(HPLC)测定其对血管紧张素转化酶(ACE)的抑制活性,采用DPPH自由基清除法、氧自由基清除能力(ORAC)法研究其抗氧化性。结果表明:草鱼肽抑制ACE活性IC5 0为0.229mg/mL,清除DPPH自由基的IC50为7.630mg/mL,ORAC值为1317.962μmol Trolox/g,采用草鱼制备有效的生理活性肽是可行的。In this study,bioactive peptides were prepared from grass carp meat by enzymatic hydrolysis and ultrafiltration.Their angiotensin I-converting enzyme（ACE） inhibitory activity was determined by high performance liquid chromatography（HPLC） and antioxidant activity was evaluated by DPPH radical scavenging and ORAC（oxygen radical absorbance capacity） assays.The results showed that the prepared bioactive peptides had strong ACE inhibitory activity with IC50of 0.229 mg/mL.The concentration needed to scavenge 50% DPPH radicals was 7.630 mg/mL and an ORAC value of 1317.962 Trolox μmol/g was achieved.Thus,grass carp meat is a good source of ACE inhibitory peptides.

关 键 词：草鱼肽 ACE抑制活性 DPPH自由基 ORAC 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]