复合酶法提取海带多糖工艺优化  

Optimization of Multi-Enzyme Hydrolysis of Laminaria japonica for Polysaccharide Extraction

作　　者：翟为[1] 张美双[1] 张莉霞[1] 赵岩[1] 吴鹏 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]九江中星联生物技术有限公司,江西九江332000

出　　处：《食品科学》2012年第18期6-9,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAI03B01);国家自然科学基金面上项目(31070316)

摘　　要：以海带为原料,复合酶法提取海带多糖。在单因素试验的基础上,采用正交试验及方差分析确定复合酶法提取海带多糖的最佳条件:纤维素酶0.5%、果胶酶1.0%、木瓜蛋白酶1.0%、木聚糖酶1.0%、温度30℃、时间3h、pH 5.0。在此条件下,海带中多糖的提取率为78.9%。使用复合酶前处理工艺,海带多糖产率较传统工艺提高了近3倍。This study was designed to optimize the extraction of polysaccharides from Laminaria japonica by one-step hydrolysis with four different enzymes. By using orthogonal array design and analysis of variance based on one-factor-at-a- time experiments, the optimal hydrolysis conditions for polysaccharide extraction from Laminariajaponica were 0.5% cellulase, 1.0% pectinex, 1.0% papaln, 1.0% xylanase, extraction temperature of 30 ℃, extraction time of 3 h and pH 5.0. Under these conditions, the extraction yield of polysaccharides was 78.9%, which was increased approximately 3-fold compared with that obtained by the traditional extraction method.

关 键 词：海带 复合酶 海带多糖 

分 类 号：TS201.1[轻工技术与工程—食品科学]