超高效液相色谱法测定食品中5种人工合成甜味剂  

UHPLC Determination of 5 Artificial Edulcorators in Food

作　　者：肖立群[1,2] 张承聪[2] 张承明[1] 

机构地区：[1]云南烟草科学研究院云南烟草化学重点实验室,昆明650106 [2]云南大学化学科学与工程学院,昆明650091

出　　处：《理化检验（化学分册）》2012年第12期1427-1429,1432,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：提出了超高效液相色谱法测定食品中5种人工合成甜味剂安赛蜜、糖精钠、阿斯巴甜、甘素和纽甜含量的方法。详细叙述了不同样品的前处理方法,以MN NUCLEODUR C18Pyramid(3mm×250mm,5.0μm)色谱柱为固定相,以不同体积比混合的pH 6.0的5.0mmol.L-1乙酸-乙酸铵缓冲溶液和甲醇为流动相进行梯度洗脱,采用二极管阵列检测器于波长235nm处进行测定。5种甜味剂的质量浓度分别在一定的范围内与峰面积呈线性关系,安赛蜜、糖精钠、阿斯巴甜、甘素和纽甜的检出限(3S/N)依次为0.476,0.005,3.590,0.002,219.9mg.L-1。加标回收率在75.0%～104%之间;测定值的相对标准偏差(n=5)在0.3%～7.2%之间。UHPLC was applied to the determination of 5 artificial edulcorators, i. e. , acesulfame, sodium saccharin, aspartame, dulcin and neotame in food. Methods for pretreatment of different kinds of samples were described in detail. MN NUCLEODUR C18 Pyramid column （3 mm× 250 mm, 5. 0 μm） was used as stationary phase, and a mixed solution of 5. 0 mmol · L^-1 acetic acid ammonium acetate buffer solution of pH 6. 0 and methanol in different ratios was used as mobile phase for gradient elution, and PDA detector at the wavelength of 235 nm was used for determination. I.inear relationships between values of peak area and mass concentration of the 5 edulcorators were kept in definite ranges. Values of detection limits （3S/N） of the method were found to be 0. 476, 0. 005, 3. 590, 0. 002 and 219. 9 mg · L ^-1 respectively. Tests for recovery gmd precision were made by standard addition method, values of recovery were ranged from 75. 0% to 104% and RSD＇s （n=5） found were ranged from 0. 3 % to 7.2%. Keywords： UHPI.C; Edulcorators; Acesulfame; Sodium saccharin; Aspartame; Dulcin; Neotame; Food

关 键 词：超高效液相色谱法 甜味剂 安赛蜜 糖精钠 阿斯巴甜 甘素 纽甜 食品 

分 类 号：O657.63[理学—分析化学]