豆渣膳食纤维及豆渣超微化制品对小鼠肠道菌群的影响  

Effect of Dietary Fiber from Okara and Superfine Okara on Intestinal Microflora in Mice

作　　者：吴占威[1] 胡志和[1] 邬雄志[1] 

机构地区：[1]天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津300134

出　　处：《食品科学》2013年第3期271-275,共5页Food Science

摘　　要：用豆渣膳食纤维及豆渣超微化制品灌胃BALB/c小鼠,研究其对小鼠肠道菌群的影响。正常对照组用生理盐水进行灌胃,实验组分别以低剂量(0.5g/(kg.d))、高剂量(2.5g/(kg.d))灌胃水溶性膳食纤维(SDF)、非水溶性膳食纤维(IDF)、超微粉碎豆渣(SPO)和螺杆挤压-超微粉碎豆渣(ESPO)。实验期间,每周同一时间取小鼠粪便,采用选择性培养基检测小鼠粪便中乳酸杆菌、双歧杆菌、肠杆菌和肠球菌的数量。结果表明:灌胃的各个阶段,各实验组在灌胃豆渣膳食纤维及豆渣制品后对小鼠的肠道菌群均有明显影响,其中高剂量IDF、低剂量SDF和低剂量的ESPO对小鼠肠道菌群调理作用明显,在增加乳酸杆菌和双歧杆菌的同时,一定程度上抑制了肠球菌和肠杆菌的增长。In order to explore the effect of dietary fiber from okara and superfine okara on intestinal microflora of mice, BALB/c mice were subjected to gavage treatments. The mice from control group were administered with normal saline, and the mice from experimental groups were administered with soluble dietary fiber （SDF）, insoluble dietary fiber （IDF）, superfine pulverization okara （SPO） and extrusion-superfine pulverization okara （ESPO）, respectively, at low （0.5 g/（kg d）） and high （2.5 g/（kg d） doses. During experiments, the selective medium was used to detect the quantity of Lactobacillus, Bifidobacterium, Enterobacter and Enterococcus in the fecal of mice at the same time every week. The results showed that each treatment group was susceptible to intestinal microflora after administration of dietary fiber from okara and superfine okara at the early stage. The high-dose IDF, low-dose SDF and low-dose ESPO had obvious regulation effect on intestinal microflora of mice with the obvious enhancement of Lactobacillus and Bifidobacterium, and the suppression of Enterobacter and Enterocoecus.

关 键 词：豆渣 膳食纤维 肠道菌群 超微粉碎 螺杆挤压-超微粉碎 

分 类 号：TS209[轻工技术与工程—食品科学]