木聚糖酶对苹果肉渣膳食纤维改性的研究  

Research of the dietary fiber modified from apple flesh pomace using xylanase enzyme

作　　者：付成程[1] 郭玉蓉[1] 严迈[1] 霍天博[1] 薛战锋[1] 孙迪迪[2] 贾莉[2] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710062 [2]陕西师范大学化学化工学院,陕西西安710062

出　　处：《食品工业科技》2013年第7期140-143,152,共5页Science and Technology of Food Industry

基　　金：苹果产业技术体系(CARS-28)

摘　　要：以苹果肉渣为原料,通过木聚糖酶酶解,确定最优工艺,并研究所得水溶性膳食纤维及分离滤渣的理化性质。结果表明:采用木聚糖酶法,得到的水溶性膳食纤维提取率为19.58%。酶解温度55℃,酶解pH7.0时其最适反应条件为:酶添加量12.375U/g、酶解时间7h。酶解所得水溶性膳食纤维有较高的溶解性,表观粘度有所降低;分离滤渣的持水力与膨胀力均提高;红外光谱分析显示,酶解所得水溶性膳食纤维以及分离滤渣均产生糖的特征吸收峰,而在电镜扫描下,超微结构变化明显。Study on the optimal process and determine physical and chemical properties of soluble dietary fiber and separated residues,with the apple flesh pomace as raw materials which were hydrolysed by xylanase.The results showed that the yield of soluble dietary fiber was 19.58% processed by xylanase.The optimal reaction conditions were as follows： the enzyme addition was 12.375U/g, reaction time was 7h, reaction temperature was 55~C, and reaction pH was 5.0.Water-soluble dietary fiber gained by xylanase had higher solubility and lower apparent viscosity than raw materials.The retention ability and expansibility of the separated residues were improved.The infrared spectrum analysis described both soluble dietary fiber and separated residues illustrated similar characteristic absorption peak as polysaccharide,accompanied by obvious changes of microstructure which could be seen under scanning electron microscope.

关 键 词：苹果肉渣 木聚糖酶 膳食纤维 改性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]