复合酶降解壳聚糖研究  

Study on the degradation of chitosan by compound enzyme

作　　者：王宇[1] 叶盛权[1] 姜秋焕[1] 

机构地区：[1]广东海洋大学食品科技学院/广东省水产品加工与安全重点实验室/广东普通高等学校水产品深加工重点实验室,广东湛江524088

出　　处：《广东农业科学》2013年第5期95-98,共4页Guangdong Agricultural Sciences

基　　金：广东省教育部产学研结合项目(2008B090500002);广东海洋大学引进人才科研启动基金(1010295);湛江市财政资金科技专项竞争性分配项目重大科技专项(湛科[2012]86号)

摘　　要：以木瓜蛋白酶和纤维素酶对壳聚糖进行降解研究,分别考察温度、pH值、底物浓度、酶底比以及复合酶配比对降解过程的影响,然后通过红外光谱分析降解产物的结构。结果表明:最佳酶解条件为pH 5.0、温度45℃、底物浓度12 g/L、酶底比6%、复合酶配比2:1。红外分析表明酶解前后壳聚糖化学结构没有发生改变。This paper described the enzymatic hydrolysis of chitosan by papain and cellulase. The effects of temperature, pH, substrate concentrations, the ratio of emzyme to substrate and the proportion of compound enzyme on the degradation of chitosan were investigated, and the infrared spectrum was used to analyse the structure of degradation products. The results showed that the optimal pH, temperature, concentration of substrate, the ratio of emzyme to substrate and the proportion of compound enzyme were pH 5.0, 45℃, 12 g/L, 6% and 2：1 respectively. Infrared analysis indicated that the chemical structure of chitosan was not changed after enzymolysis.

关 键 词：壳聚糖 酶解 木瓜蛋白酶 纤维素酶 红外光谱 

分 类 号：O636.1[理学—高分子化学]