正交试验优化鼠曲草色素提取及其稳定性  

Extraction Optimization by Orthogonal Array Design and Stability of Pigment from Herba Gnaphaii Affinis

作　　者：丁月[1] 徐超[1] 陈霞霞[1] 郑易林[1] 万力婷[1] 陈伊梦[1] 王鸿飞[1] 

机构地区：[1]宁波大学食品科学与工程系,浙江宁波315211

出　　处：《食品科学》2013年第10期103-106,共4页Food Science

基　　金：宁波大学SRIP项目(2011T29)

摘　　要：以鼠曲草为试验原料,采用浸提法提取鼠曲草色素,分别研究乙醇体积分数、料液比及浸提时间等因素对提取效果的影响,在单因素试验的基础上,通过L9(34)正交试验优化提取参数。同时考察温度、光照、溶液pH值及金属离子对鼠曲草色素稳定性的影响。结果表明,鼠曲草色素最优提取工艺为乙醇体积分数90%、料液比1:300(g/mL),浸提时间100min。鼠曲草色素的最大吸收波长为416nm,对光照和温度稳定性较好,在碱性条件下色素比较稳定,Cu2+、Ca2+及Zn2+对色素稳定性影响较大。A solvent extraction technique was developed to extract pigment from Herba Gnaphaii Affinis and optimized by investigating the effects of ethanol concentration,solid-to-liquid ratio and extraction time on extraction efficiency using onefactor-at-a-time and orthogonal array design.The stability of the pigment obtained was also investigated to temperature,light,pH and metal ions.The optimal extraction conditions were found to be 90% ethanol,a solid-to-liquid ratio of 1：300（g/mL） and 100 min.The pigment obtained under these conditions had maximum absorption at 416 nm and was stable to light,temperature and alkaline condition but greatly influenced by Cu2＋,Ca2＋and Zn2＋.

关 键 词：鼠曲草 色素 正交试验 稳定性 

分 类 号：TS264.4[轻工技术与工程—发酵工程]