酶法水解碎米制取碎米小分子多肽  

Preparation of low-molecular peptides from broken rice by enzymatic hydrolyzation

作　　者：王林[1] 胡玲[1] 郑在[1] 曹贤斌[1] 陈晓艳[1] 徐书旸[1] 

机构地区：[1]安徽大学生命科学学院,安徽合肥230601

出　　处：《食品工业科技》2013年第12期203-206,224,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(60908022;51205400);亚热带农业生物资源保护与利用国家重点实验室开放课题(KSL-CUSAb-2012-03);安徽大学"211工程"三期第三批青年科学研究基金(KJQN1125);大学生创新创业训练计划项目(cxcy2012049)

摘　　要：以碎米为原料,对蛋白酶水解碎米制取小分子多肽的工艺条件进行了研究。首先采用单因素实验确定影响多肽产率的主要因素及其取值范围。以此为基础,应用响应面法对加酶量、酶解温度、pH三因素进行优化,建立了以多肽产率为响应值的二次回归方程,获得了酸性蛋白水解碎米制取小分子多肽的最佳工艺参数:加酶量5272U/g,酶解温度54℃,pH4.26。在料液比1∶20,酶解2h条件下,多肽产率达到18.92%,接近理论预测值,表明预测模型可靠性较高,可为酶法制取碎米多肽的实际生产提供理论依据。The process conditions for low-molecular peptides prepared by enzymatic hydrolyzation from broken rice were researched. Firstly,single factor experiments were used to confirm the main factors and their value scales affecting peptide yield rate. Based on this result,response surface methodology（RSM） was applied to optimize enzyme dosage,temperature and pH,and the second-order equation model for the yield of peptides was established. The optimal parameters for peptides yielding by acid protease hydrolyzation was 5272U/g of enzyme dosage,54℃ of reaction temperature and pH4.26. With 1：20 of solid-liquid ratio and 2 hours hydrolyzing,the peptides yielding was up to 18.92%,which was nearly in agreed with the theoretical prediction value. The results suggested the predicted model was reliable and would provide some theoretic reference for the practical producing of broken rice peptides by enzymatic hydrolyzing.

关 键 词：碎米 多肽 酶法水解 工艺优化 响应面法 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]