双酶法制备小麦微孔淀粉的工艺条件优化  

Optimum Preparation Conditions for Wheat Micorporous Starch by Double Enzyme Hydrolysis Method

作　　者：张国权[1] 张兰月[1] 罗勤贵[1] 梅仕峰[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品研究与开发》2013年第12期1-5,9,共6页Food Research and Development

基　　金：现代小麦产业技术体系建设专项(MATS)

摘　　要：为丰富微孔淀粉类型、拓展小麦淀粉的应用领域,以小麦A淀粉为材料,采用α-淀粉酶与糖化酶双酶协同水解法优化小麦微孔淀粉的制备工艺条件。结果表明:影响小麦微孔淀粉吸水率的因素依次为反应温度、酶用量、反应时间和pH,影响吸油率的因素依次为反应温度、pH、反应时间和酶用量。制备小麦微孔淀粉的适宜条件为,糖化酶与α-淀粉酶的配比6∶1,淀粉乳浓度20%,反应温度55℃,酶用量1.25%,pH4.5,反应时间16 h,此条件下小麦微孔淀粉的吸水率为115.14%,吸油率为103.46%,其吸附性能远高于原淀粉。In order to enrich the microporous starch types and broaden the application fields of wheat starch,the wheat A type starch was used as material and the technological conditions of preparation of microporous starch using co-hydrolysis by α-amylase and glucoamylase were optimized.The results show that reaction temperature was the main factor affecting the water absorption rate of wheat microporous starch,followed by enzyme dosage,reaction time and pH value.While the oil absorption rate was in turn influenced by reaction temperature,pH value,reaction time and enzyme dosage.The optimum preparation conditions for wheat microporous starch were glucoamylase：α-amylase ratio of 6 ∶ 1,starch concentration of 20 %（w/v）,reaction temperature of 55 ℃,enzyme dosage of 1.25 %,pH4.5 and reaction time of 16 h.Under the condition,water absorption rate of microporous starch was up to 115.14 %,oil absorption rate was up to 103.46 %.The absorption properties of micropous starch is higher than raw starch obviously.

关 键 词：小麦A淀粉 微孔淀粉 双酶水解 工艺条件 

分 类 号：TS236.9[轻工技术与工程—粮食、油脂及植物蛋白工程]