不同分子量水溶性大豆多糖铁(Ⅲ)配合物的合成及其抗氧化活性研究  

Study on synthesis and antioxidant activity of different molecular weight soluble soybean polysaccharide-Fe(Ⅲ) complex

作　　者：万真真[1] 高文宏[1] 曾新安[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品工业科技》2013年第16期109-113,共5页Science and Technology of Food Industry

基　　金：广东省科技计划项目(2012A020200002);广东省领军人才项目

摘　　要：研究了超声波协同过氧化氢氧化制备低分子量水溶性大豆多糖,得到了4种不同分子量的水溶性大豆多糖,其分子量分别为93.9、43.4、18.8、9.6ku,并结合超滤从水溶液大豆多糖原液分离出分子量为155、2.6ku的两种多糖。将6种多糖分别与Fe3+反应合成不同分子量的水溶性大豆多糖-Fe(Ⅲ)配合物[SSPS-Fe(Ⅲ)],在还原力、羟基自由基、脂质过氧化、亚硝酸盐自由基四种不同的体系下进行SSPS-Fe(Ⅲ)的体外抗氧化活性研究。结果表明,不同分子量SSPS-Fe(Ⅲ)均有抗氧化活性,其中分子量最大的SSPS-Fe(Ⅲ)的抗氧化活性较弱,而分子量为9.6ku的SSPS-Fe(Ⅲ)整体上具有较强的还原能力、清除羟基自由基和亚硝酸盐自由基和抑制脂质过氧化的能力,表明SSPS-Fe(Ⅲ)的抗氧化能力与其相对分子质量大小有关。Four different low -molecular -weight soluble soybean polysaccharides were prepared by hydrogen peroxide with ultrasonic treatment. They were obtained with molecular weights of 93.9,43.4,18.8 and 9.6ku. Two soluble soybean polysaccharides （SSPS） were obtained with molecular weights of 155 and 2.6ku when material SSPS solution was ultrafiltrated. Subsequently every different molecular weight SSPS reacted with Fe 3＋ to form its ferrous complex （SSPS-Fe （ Ⅲ ） ）. Antioxidant activities of six SSPS-Fe （ Ⅲ ） complexes were evaluated in vitro by total reducing power,hydroxyl free radical scavenging capacity,sodium nitrite free radical scavenging capacity , and lipid peroxidation inhibition capacity . Results showed that six SSPS-Fe （ Ⅲ ） complexes had different antioxidant activities. Compared with five SSPS-Fe （Ⅲ） complexes,the one with the highest relative molecular mass had weaker antioxidant activity. The SSPS-Fe （Ⅲ ） complex with molecular weight of 9.6ku performed the strongest reducing power,scavenging activities to hydroxyl and sodium nitrite free radical,and lipid peroxidation inhibition. Consequently the antioxidant potential of SSPS-Fe（Ⅲ） complexes was presumably related to their relative molecular mass.

关 键 词：水溶性大豆多糖 铁(Ⅲ)配合物 合成 抗氧化活性 低分子量 

分 类 号：TS201.2[轻工技术与工程—食品科学]