枯草芽孢杆菌BS08对大黄鱼腐败菌的抑制及培养基优化  

Inhibitory Effect of Bacillus Subtilis BS08 on Spoilage Bacteria Isolated from Pseudosciaena Crocea and Optimization of Fermentation Medium for BS08

作　　者：张雯[1,2] 倪莉[2] 陈雅静[2] 叶秀云[1,2] 

机构地区：[1]福州大学化学化工学院,福州350108 [2]福州大学生物科学与工程学院食品科学技术研究所,福州350108

出　　处：《中国食品学报》2013年第8期102-109,共8页Journal of Chinese Institute Of Food Science and Technology

摘　　要：对峙试验证实枯草芽孢杆菌BS08对大黄鱼腐败菌(腐败希瓦氏菌、假单胞菌、嗜冷杆菌)有明显拮抗作用。扩散抑菌试验和发酵抑菌试验表明枯草芽孢杆菌BS08发酵滤液对嗜冷杆菌有明显的抑制作用。为提高枯草芽孢杆菌发酵液的抑菌活性,通过单因素多水平试验和Box-Behnken响应面试验,优化BS08的培养基。优化培养基中最佳碳源、氮源和无机盐分别为葡萄糖、酵母粉、MgSO4,3种营养成分的添加量分别为14,14,1 g/L。37℃摇瓶发酵培养48 h后制备发酵滤液,添加至嗜冷杆菌液体培养基中,添加量为20%,培养24 h时对嗜冷杆菌的抑制率达67.2%,比优化前的抑菌率提高了20.8%。Obvious antagonistic effects of Bacillus subtilis BS08 against spoilage bacteria （Shewanella putrefaciens, Pseudomonas fluorescens, Psychrobacter irnmobilis ） isolated from Pseudosciaena crocea were found by the dural culture methods. Fermentation filtrate of Bacillus subtilis BS08 showed strong antibacterial activity on Psychrobacter immobilis. Single factor experiments and Response surface methodology were used to optimize the fermentation medium for production of antibacterial substances by BS08. The results showed that the optimal medium ingredients were comprised of glucose 14 g/L, yeast extract 14 g/L, MgSO4 1 g/L,with 20% additive volume of fermentation filtrate, and the bacteriostasis rate reached 67.2% after incubation for 24 h, improved 20.8% than using initial fermentation filtrate.

关 键 词：枯草芽孢杆菌 大黄鱼 抑菌活性 培养基优化 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]