ε-多聚赖氨酸产生菌的筛选及16SrDNA测序鉴定  

Screening of ε-poly-l-lysine producing strains and molecular identification by 16S rDNA sequence

作　　者：黄莉[1,2] 唐仁勇[1,3] 张佳敏[1,3] 王亚[1] 王卫[1,3] 刘达玉[1,3] 苟兴华[1,2,3] 

机构地区：[1]成都大学生物产业学院,四川成都610106 [2]西华大学生物工程学院,四川成都610039 [3]食品加工四川省高校重点实验室,四川成都610106

出　　处：《食品工业科技》2013年第17期163-167,172,共6页Science and Technology of Food Industry

基　　金：食品加工四川省高校重点实验室开放基金项目(12-508);四川省科技厅应用基础研究项目(2013JY0118)

摘　　要：利用ε-多聚赖氨酸(ε-PL)与亚甲基蓝在静电排斥的作用下能够形成透明圈的性质,初步筛选出10株产ε-PL的菌。然后摇瓶发酵复筛,根据发酵上清液与DR(dragendorff)试剂反应和纸层析两者均成阳性反应,以及参照Itzhaki方法计算发酵产物中ε-PL产量,从而筛选出编号为4#、8#、Y#的三株产量较高的菌株,其ε-PL产量分别达到0.757、0.751、0.808g/L。再采用基于16S rDNA序列分析和系统发育分析的方法,对4#、8#、Y#三株菌进行分子生物学鉴定,最终确定4#可能是Delftia acidovorans,8#可能是Stenotrophomonas maltophilias,Y#可能是Streptomyces griseolus。ε-poly-L-lysine （ε-PL） was widely used as food preservative. In th is research,10 ε-poly-L-lysi ne （ε-PL） production strains were obtained by special zoon formation due to electrostatic interaction between ε-poly-L- lysine（ε-PL） and methylene blue.Shake-flask cultures were followed for screening of high producing strains.The results from the reaction of the supernatant and dragendorff reagent and from the paper chromatography were both positive,and then the Itzhaki method was referred to calculate the yield of ε-PL in the fermentation broth to screen out the highest ε-PL production strains from these 10 candidate strains.Finally,3 production strains were obtained, which were numbered as 4^#, 8^# and Y^#, whose ε-PL production reached 0.157, 0.751, 0.808g/L respectively.Molecular identification by 16S rDNA sequence analysis in strain 4^#, 8^# and Y^# was performed and then system phylogenetic analysis were carried out.The results indicated the strain 4^# might be Delftia acidovorans,the strain 8^# might be Stenotrophomonas maltophilia, and the strain Y^# miqht be Streptomyces griseolus.

关 键 词：筛选 ε-多聚赖氨酸 16S rDNA测序分析 系统发育分析 

分 类 号：TS201.3[轻工技术与工程—食品科学]