猪肉、牛肉和绵羊肉掺伪PCR的检测技术  

PCR on Adulteration Detection of Pork, Beef and Mutton

作　　者：徐瑗聪 董凯[1,2] 黄昆仑[1,3] 许文涛[1,3] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京福德安科技有限公司,北京100083 [3]农业部转基因生物使用安全检验监督测试中心,北京100083

出　　处：《农业生物技术学报》2013年第12期1504-1508,共5页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863)(No.2012AA101606)

摘　　要：肉(制)品的掺杂的假现象日益泛滥,传统的检测方法成本较高、精确度低,无法实现快速简便的实时检测,一种简便、高效和快速的检测方法亟待开发。本研究通过传统的PCR方法对引物进行筛选、验证,针对猪、牛和绵羊肉类分别选出了一对高特异性引物,分别对猪、牛和绵羊肉基因组DNA进行实时荧光定量PCR扩增,检测限分别为1.69、1.52和17.5拷贝;利用此引物进一步开发为肉类掺假检测试剂盒,试剂盒对市场上的深加工肉制品进行检测的结果表明,能够有效地鉴别出猪、牛和绵羊肉制品。该试剂盒操作简单,不需要专业人员,在现场检测中有很好的应用前景。With more and more appearence of meat adulteration, one simple, high-effency and quick detection method is urgent which compares to tradional ones with expensive, low-accuracy and low-efficiency. In this study, adulteration detection with molecular biology techniques was researched. A pair of highly specific primers for pork, beef and sheep were obtained, respectively, by trational PCR screening and verification. The Real-time fluorescence quantitative PCR results of pork, beef and sheep with 3 primer combinations showed that the detection limits were 1.69 copy, 1.52 copy and 17.5 copy, respectively. The 3 primer combinations were then developed into detection kits for pork, beef and mutton. The kits developed in this study identified pork, beef and mutton effectively when they were used to detect several deep-processed meat products in market. The kit is simple, which shows a good application prospect in field use.

关 键 词：猪肉 牛肉 绵羊肉 掺伪 PCR 

分 类 号：TS207.3[轻工技术与工程—食品科学]