丝胶多肽的分离及其抗氧化活性研究  

Preparation,Separation and Antioxidant Activity of Peptides Derived from Silk Sericin

作　　者：范金波[1] 周素珍[1] 任发政[2] 郑立红[3] 姜鹭[2] 冯叙桥[1] 

机构地区：[1]渤海大学化学化工与食品安全学院,辽宁锦州121013 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]河北科技师范学院食品工程系,河北昌黎066000

出　　处：《中国食品学报》2013年第12期46-51,共6页Journal of Chinese Institute Of Food Science and Technology

摘　　要：以家蚕丝胶为原料,采用Alcalase碱性蛋白酶水解技术制备丝胶抗氧化肽。经分子质量膜截留、分子筛及离子交换层析等手段分离活性肽成分,并对水解液及分离组分进行抗氧化活性研究。结果表明:通过超滤获得4组不同分子质量范围的肽段,其中P4(MW<3 ku)ABTS自由基清除活性显著高于其它3组(P<0.05),其IC50值为29.30 mg trolox eq/g;P4肽段经Sephadex G-25凝胶层析、SP-Sephadex C-25阳离子交换分离,得到强活性肽段SP2和SP3,其清除ABTS自由基的IC50值分别为156.25 mg trolox eq/g和144.23 mg trolox eq/g。该研究为开发丝胶抗氧化肽功能产品提供了理论依据,为合理开发和利用丝胶提供了新的途径。The objectives of this study were to prepare silk sericin hydrolysates（SSH） from sericin of silkworm（Bombyx mori）by Alcalase enzymatic hydrolysis,separation and isolation of the activity peptides from the hydrolysate by a series of separation and analysis technology,including ultrafiltration,gel filtration chromatography and ion-exchange chromatography.The antioxidant activities of hydrolysates was evaluated in vitro.The hysdrolytes were separated by Ultra-filtration and collected to make four groups according to molecular weight,P4（MW 3ku） exhibited the highest free radical scavenging activity（P〈0.05）,IC50 value was 29.30 mg trolox eq/g.Group P4 was further separated by gel filtration chro-matography and ion-exchange chromatography,which produced two major fractions：SP2 SP3.Their IC50 value of ABTS cation radical scavenging activity were 156.25 mg trolox eq/g and 144.23 mg trolox eq/g,respectively.The work will be a base for development of antioxidative functional food production and provide a method for potential exploitation and uti-lization of silk sericin.

关 键 词：丝胶 酶解 多肽 分离 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学] R285.5[轻工技术与工程—食品科学与工程]