南瓜多糖对不同糖尿病模型小鼠的降糖作用  

The Hypoglycemic Effect of the Pumpkin Polysaccharide to the Different Diabetic Model Mice

作　　者：张高帆[1] 苏东洋[1] 张拥军[1] 金晖[1] 陈萍[1] 朱丽云[1] 王为民[1] 王革[1] 

机构地区：[1]中国计量学院生命科学学院,杭州310018

出　　处：《中国食品学报》2014年第2期23-27,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省自然科学基金项目(LY13C200007)

摘　　要：目的:研究南瓜多糖对不同试验性高血糖模型小鼠血糖的影响,并研究其降血糖作用机制。方法:降血糖试验采用四氧嘧啶、肾上腺素、地塞米松3种糖尿病模型,对试验小鼠灌胃PPS 200 mg/kg,观察不同给药时间小鼠的血糖、肝糖原、胰脏胰岛素等指标。结果:PPS对四氧嘧啶所致糖尿病小鼠的血糖升高有显著的抑制作用(P<0.01),胰脏胰岛素水平与模型组比较显著增加(P<0.05),肝糖原值保持正常水平;PPS能够显著降低肾上腺素模型小鼠的高血糖(P<0.01);同时,PPS能够降低由地塞米松引起的糖尿病小鼠口服糖负荷后血糖的峰值,加快血糖水平回落的速度。结论:PPS可对抗四氧嘧啶引起的小鼠血糖升高,增加小鼠的肝糖元合成,改善糖耐量水平,其作用机制可能与改善胰岛β细胞的功能或增加肝组织对糖的转化利用有关。Objective： To evaluate the hypoglycemia activity of the pumpkin polysaccharide （PPS） on different experimental diabetic model mice, and elucidate the possible mechanism of its hypoglycemic effects. Methods： Alloxan, a- drenalin and dexamethasone were used to induce hyperglycemia models in mice, followed by treatment them with PPS at the dose of 200 mg/kg by intragastric administration. The serum glucose, glycogen, and insulin in mice were detected. Results： Compared with that of the diabetic model group, PPS could significantly reduce serum glucose in mice（P〈0.01） induced by alloxan. Also, it was observed that PPS could significantly increase serum insulin level in mice（P〈0.05）, and kept liver glycogen 6n normal level. PPS could inhibit the increase of serum glucose in mice （P〈0.01） induced by a- drenalin. Meanwhile, PPS could reduce the peak value of serum glucose induced by dexamethasone and speeded up restoration of the increased serum glucose level. Conclusion： The PPS can significantly inhibit the alloxan-induced rise of serum glucose, improve glucose tolerance and increase hepatic glucogen synthesis. The potential mechanism of reducing serum glucose was associated with its rehabilitating injured β-cells in the islet or increasing tissue transformation and glucose utilization.

关 键 词：南瓜多糖 四氧嘧啶 肾上腺素 地塞米松 降血糖作用 

分 类 号：R587.1[医药卫生—内分泌]