液质联用法测定黄酒中环匹阿尼酸  

Detection of Cyclopiazonic Acid(CPA) in Yellow Rice Wine by HPLC-MS

作　　者：彭祺[1] 王芳[1] 李岩冰[1] 吴坚[1] 郑云峰[1] 张弘[1] 李博斌[1] 

机构地区：[1]国家黄酒产品质量监督检验中心,浙江绍兴312071

出　　处：《酿酒科技》2014年第4期86-88,共3页Liquor-Making Science & Technology

基　　金：浙江省质量技术监督系统科研计划项目(20100234)

摘　　要：采用高效液相色谱一三重四级杆质谱串联法（HPLC—MS／MS）技术，结合碱性甲醇水溶液及正己烷脱脂提取．乙酸铵水溶液与乙腈配比做流动相洗脱法，建立检测黄酒中环匹阿尼酸的分析方法。结果表明，液质联用法对黄酒中环匹阿尼酸检测限为50ug／kg，定量限为200ug／kg，线性范围为25～1050ug／kg（R〉0．999），回收率在80．1％～85．1％之间，相对标准偏差（RSD）在6．2l％～9．17％之间。液质联用法可对黄酒中环匹阿尼酸进行准确的定性和定量，且避免了对样品费时繁复的提取过程，提高了大批量样品检测前处理的效率，同时解决了检测中出现假阳性的问题，适合黄酒中环匹阿尼酸低残留检测要求。High performance liquid chromatography-triple quadruple mass spectrometry （HPLC-MS）, coupled with alkaline methanol solu- tion＋n-hexane extraction and ammonium acetate solution＋acetonitrile mobile phase elution, were applied to detect cyclopiazonic acid in yellow rice wine. The experimental results suggested that, the detection limit was 50 ixg/kg, the quantification limit was 200 ixg/kg, the linear range was between 25 - 1050 ug/kg （R 〉 0.999）, the recovery was between 80.1%- 85.1%, and RSD was within the range of 6.21%-9.17 %. The appli- cation of such method could achieve accurate qualitative and quantitative analysis of CPA in yellow rice wine, avoid time-consuming and trouble- some sample extraction, improve greatly sample treatment efficiency especially for samples on a large scale, and settle the problem of false posi- tive in the detection. Besides, it met the detection requirements of low residue of CPA in yellow rice wine.

关 键 词：三重四级杆 质谱 黄酒 环匹阿尼酸 

分 类 号：O657.63[理学—分析化学] TS262.4[理学—化学]