构建直接发酵淀粉产生酒精的酵母融合菌株的研究  

Construction of Yeast Fusants of Directly Transform Starch into Ethanol

作　　者：庞小燕[1] 王吉瑛[2] 赵凤生[1] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240 [2]兰州大学生命科学学院,兰州730000

出　　处：《生物工程学报》2001年第2期165-169,共5页Chinese Journal of Biotechnology

摘　　要：以酒精酵母和热带假丝酵母为亲本 ,通过单亲灭活原生质体融合技术 ,获得了既具有糖化酶活性又能高产酒精的稳定的酵母融合株 ,并测定和比较了融合子的细胞大小、DNA含量以及比增长率 μ、淀粉利用能力、乙醇耐受性、α 淀粉酶和糖化酶活力等生产性能。属间原生质体融合率为 9 2× 10 -6。F 1和F 5两株融合子性状较好 ,酒精发酵产量可达 8 8%和 11 5 % ,具有良好的应用前景。To obtain strains that are able to efficiently produce ethanol directly from starchy material, we have used the protoplast fusion technique to construct hybrids between \%Saccharomyces cerevisiae\% and \%Candida tropicalis\%. The isolation of fusants used a selective system by a irreversible biochemical inhibitor\|Iodoacetic Acid(ID), and the fusion frequency was 9.2×10 -6 . The cell size and DNA content of the fusants were determined. Through testing some productive properties such as specific growth rate, saccharification ability, ethanol tolerance and the activities of α amylase, saccharogen amylase, we selected two fusants\_F\|1 and F\|5. They were more superior and their ethanol production came up to 8.8% and 11.5%.

关 键 词：酒精酵母 热带假丝酵母 原生质体融合 酒精发酵 发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]