HCV-specific cytokine induction in monocytes of patients with different outcomes of hepatitis C  

作　　者：Rainer P.Woitas Uwe Petersen Dirk Moshage Hans H.Brackmann Bertfried Matz Tilman Sauerbruch Ulrich Spengler 

机构地区：[1]Department of Intemal Medicine I,University of Bonn,53105 Bonn,Germany [2]Institute of Experimental Hematology,University of Bonn,53105 Bonn,Germany [3]Institute of Medical Microbiology and Immunology,University of Bonn,53105 Bonn,Germany

出　　处：《World Journal of Gastroenterology》2002年第3期562-566,共5页世界胃肠病学杂志（英文版）

基　　金：a generous grant of the Joachim Kuhlinann AIDS-Stiftung

摘　　要：AIM:Cytokine release by macrophages critically determines the type of immune response to an antigen.Therefore,we studied hepatitis C virus(HCV)-specific induction of interleukins-1β,-10,-12(IL-1β,IL-10,IL-12),and tumor necrosis factor-α(TNF-α)in monocytes. METHODS:Intracallular cytokine expression was studied by flow cytometry in 23 patients with chronic hepatitis C,14 anti-HCV seropositives without viremia and 11 controls after stimulation of peripheral blood mononuclear calls with recombinant core,NS3,NS4,NSSa and NSSb proteins. RESULTS:Patients with HCV viremia revealed greater spontaneous expression of IL-1β,TNF-α,and IL-10. Furthermore,greater than twofold higher IL-10 expression was induced by the HCV antigens in chronic hepatitis C than in the other two groups(P<0.05).In contrast,neither IL- 12 nor TNF-α was induced preferentially. CONCLUSION:In chronic hepatitis C antigen-specific cytokine induction in monocytes is apparently shifted towards predominant IL-10 induction-not counterbalanced by antiviral type 1 cytokines.This may contribute to persistent viral replication.AIM：Cytokine release by macrophages critically determines the type of immune response to an antigen Therefore.we studied hepatitis C virus (HCV0-Specific induction of interleukins-1β,-10,-12(IL-1β,il-10,IL-12),and tumor necrosis factor-α(TNF-α) in monocytes.METHODS:Intracellular cytokine expression was studied by flow cytometry in 23 patients with chronic hepatitis C,14 anti-HCV seropositives without viremia and 11 controls after stimulation of peripheral blood mononuclear cells with recombinant core,NS3,NS4 NS5a and NS5b proteins .RESULTS:Patients with HCV viremia revealed greater spontaneous exprssion of IL-1β,TNF-α,and IL-10,Furthermore,greater than twofold higher IL-10 epression was induced by the HCV antigens in chronic hepatitis C than in the other two groups (P<0.05) In contrast,neither IL-12 noir TNF-α was induced preferentially.CONCLUSION:In chonic hepatitis C antigen-specific cytokine induction in monocytes is apparently shifted towards predominant IL-10 induction-not counterbalanced by antiviral type 1 cytokines,This may contribute to persistent viral replication.

关 键 词：Adult Cytokines Female HEPACIVIRUS Hepatitis C  Chronic Humans In Vitro INTERLEUKIN-1 INTERLEUKIN-10 INTERLEUKIN-12 Male Middle Aged MONOCYTES Research Support  Non-U.S. Gov't Tumor Necrosis Factor-alpha VIREMIA 

分 类 号：R512.63[医药卫生—内科学]