复合酶法提取山葡萄渣中可溶性膳食纤维的工艺优化  

Optimizing extraction process of soluble dietary fiber from wild grape pomace using compound enzymatic method

作　　者：刘欢[1] 何文兵[1] 朱柏霖 

机构地区：[1]长白山食品工程研究中心,通化师范学院制药与食品科学学院,通化134000

出　　处：《食品科技》2014年第7期242-247,共6页Food Science and Technology

基　　金：吉林省科技发展计划项目(20130522093JH);吉林省教育厅"十二五"科学技术研究项目(2013494)

摘　　要：以山葡萄酿酒废弃物为原料,酶法提取可溶性膳食纤维。采用Box-Behnken设计方法对纤维素酶用量、半纤维素酶用量、提取时间和提取温度4个单因素工艺参数进行优化,用响应面分析法确定4个因素的最佳工艺条件,建立数学模型。结果表明:纤维素酶用量为93 IU/g、半纤维素酶用量为121 IU/g、提取时间为257 min和提取温度为51℃,在此条件下进行验证试验,可溶性膳食纤维提取率为152.13 mg/g。山葡萄渣可溶性膳食纤维功能特性的试验结果表明,对DPPH自由基的清除率超过50.31%,膨胀力和持水力分别为5.57 mL/g和4.91 g/g。Soluble dietary fiber was prepared from wild grape pomace using enzymatic method. Based on Box-Behnken design, cellulase concentration, hemicellulase concentration, extraction time and extraction temperature were chosen as the four important factors with three levels. The process conditions were optimized by response surface method, and a mathematical model was setted up. The optimized parameters for soluble dietary fiber extraction were as follows： cellulase concentration 93 IU/g, hemicellulase concentration 121 IU/g, extraction time 257 min and extraction temperature 51 ℃. The yield rate of soluble dietary fiber reached 152.13 mg/g. The function properties of soluble dietary fiber from wild grape were investigated. Scavenging rate of DPPH radicals exceeded 50.31%. The swelling capacity was 5.57 mL/g and water holding capacity was 4.91 g/g.

关 键 词：山葡萄渣 可溶性膳食纤维 纤维素酶 半纤维素酶 响应面法 

分 类 号：TS209[轻工技术与工程—食品科学]