毛细管液相色谱测定食品中对羟基苯甲酸酯类防腐剂  

Determination of parahydroxybenzoate esters in foods by capillary liquid chromatography

作　　者：郭启雷[1] 钱聪[1] 潘红艳[1] 赵丽[1] 杨红梅[1] 

机构地区：[1]国家食品质量安全监督检验中心,北京100094

出　　处：《食品科技》2014年第9期320-323,共4页Food Science and Technology

基　　金：北京市科技计划项目(Z111100056811006)

摘　　要：建立了利用毛细管液相色谱测定食品中7种对羟基苯甲酸酯类防腐剂的分析方法。牛乳、乳饮料及植物蛋白饮料采用乙腈超声提取,酱油样品采用90%甲醇水溶液超声提取,提取液经反相毛细管液相色谱柱分离,流动相乙腈-水梯度洗脱,泵流速0.05 mL/min,紫外检测器254 nm检测。该方法在1.0～100μg/mL质量浓度范围内线性关系良好（r^2≥0.999）,在液体乳、乳饮料和植物蛋白饮料中的定量限为2.5 mg/kg,在酱油中的定量限为10 mg/kg,加标回收率为75.2%～94.1%,相对标准偏差小于6%。该方法操作简单、经济、绿色环保,为食品中对羟基苯甲酸酯的检测提供了新的技术手段。An effective and convenient method for determination of parahydroxybenzoate esters in foods by capillary liquid chromatography with ultra-violet detection was developed. Milk and protein beverage samples were extracted with acetonitrile and soysauce extracted with 90% methanol solution. The separation was performed on a reversed phase C18 capillary column in gradient elute mode with 254 nm as the UV detection wavelength. The mobile phase was composed of acetonitrile and water, at a pump flow rate of 0.05 mL/min. Under the optimal conditions, the linear range of melamine was 1.0-100 μg/mL with correlation coefficient higher than 0.999, and the limit of detection were range 2.5-10 mg/kg. The mean recoveries were 75.2%-94.1% and the relative standard deviations （RSD） were less than 6%.

关 键 词：毛细管液相色谱 食品 对羟基苯甲酸酯 

分 类 号：TS202.3[轻工技术与工程—食品科学]