高产黄色素红曲霉菌株的诱变选育  

Mutation Breeding of Monascus with High-yielding Yellow Pigment

作　　者：赵建博[1] 王伟平[1] 李改[1] 曾乐平[1] 杨建[1] 

机构地区：[1]湖北工业大学生物工程学院,武汉430068

出　　处：《中国调味品》2014年第9期4-6,共3页China Condiment

基　　金：国家自然科学基金资助项目(31101349);2012年地方高校国家级大学生创新创业训练计划项目(201210500050)

摘　　要：以红曲霉菌株Monascus SE01为出发菌株,利用紫外线诱变、双氧水筛选和氯化锂复合诱变的方法,选育得到高产黄色素的红曲霉突变株。实验结果表明:在紫外线照射60s、氯化锂质量分数为0.12%的复合诱变条件下得到的菌株SE19,经液态发酵后黄色素高达57.1U/mL,与出发菌株相比,黄色素色价提高了5.54倍。该菌株5代接种后,其黄色素产量相对稳定。To obtain the strain with high-yielding yellow pigment,Monascus SE0 1 is treated by UV mutagenesis,different concentration of hydrogen peroxide and LiCl complex mutation methods.The results show that,under the complex mutation conditions of UV radiation of 60 s and 0.12% LiCl, Monascus SE1 9 is found to be high-yielding yellow pigment producer,and its yellow pigment reaches 57.1 U/mL through liquid-state fermentation,which is 5.54 times higher than that of the start strain SE01,and the stable heritability of selected strain is still well after inoculated for 5 generations.

关 键 词：红曲霉 黄色素 复合诱变 

分 类 号：TS202.3[轻工技术与工程—食品科学]