产黄曲霉毒素B_1降解酶菌株的筛选鉴定及发酵条件优化  

Screening and identification of microorganisms producing AFB_1-degrading enzyme and optimization of fermentation conditions

作　　者：戴军[1] 邵帅[1] 王常高[1] 林建国[1] 杜馨[1] 刘秀继 姚鹃[2] 蔡俊[1] 

机构地区：[1]湖北工业大学发酵工程教育部重点实验室和工业发酵湖北省协同创新中心,湖北武汉430068 [2]安琪酵母股份有限公司,湖北宜昌443000

出　　处：《工业微生物》2014年第6期54-59,共6页Industrial Microbiology

基　　金：湖北省自然科学基金重点项目(No.2009CDA059)

摘　　要：以香豆素为唯一碳源筛选到27株能高效降解黄曲霉毒素B_1(AFB_1)的微生物菌株。用高效液相色谱检测AFB_1含量的方法进行AFB_1降解酶活力测定。以不同菌株发酵上清液中AFB_1降解酶活力高低为复筛条件,筛选到AFB_1降解酶活力最高的一株菌并命名为HSD8。该菌株经形态学、生理生化及系统发育学方法鉴定为Sinonbonas sp.。筛选所得最优菌株的发酵上清液中酶活达443 U/mL,通过单因素试验对其产酶发酵条件进行优化,以提高酶活。优化所得最佳发酵条件为:装液量50 mL/250 mL,发酵周期48 h,初始pH 5.0,接种量8%,发酵温度37 cC,摇床转速160r/min。在最佳发酵条件下,该菌株发酵上清液中酶活可达548 U/mL,比优化前提高23.7%。优选菌株HSD8在生物降解黄曲霉毒素B,方面具有应用潜力,值得进一步研究开发。In order to obtain strains for efficiently degrading aflatoxin B1 （ AFB1 ）, cumarin was used as the sole carbon source for screening. The activity of AFBl-degradation enzyme from the screened strains was determined by detecting the remnant concentration of AFB1 in fermentation liquid by using HPLC method. The strain with the highest enzyme activity was identified as Sinomonas sp. through morphological, physiological and biochemical characteristics combined with phy- logenetie analysis and was named as HSDS. Its fermentation condition was optimized using single-factor test to improve its activity of AFB^-degradation enzyme. After the strain was cultured for 48 h under the optimum conditions （50 mL/250 mL, initial pH 5.0, inoculum size 8% , 37℃ and 160 r/min） , the activity of AFBt-degradation enzyme in fermentation supernatant was elevated to 548 U/mL, which was 23. U/mL）. Strain HSD8 had application potentiality for research and development. 7% higher than that under the initial fermentation condition （433 biological degrading aflatoxin B1 and was valuable for the further,research and development.

关 键 词：黄曲霉毒素B1 降解酶 筛选 鉴定 发酵条件优化 

分 类 号：TQ920[轻工技术与工程—发酵工程]